The nucleotide sequence of a nodule-specific gene, Nms-25 of Medicago sativa

its primary evolution via exon-shuffling and retrotransposon-mediated DNA rearrangements

Zoltán Végh, Éva Vincze, Rafael Kadirov, Gábor Tóth, G. Kiss

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We present the primary structure of a nudule-specific gene, Nms-25 from Medicago sativa L. cultivar Nagyszénási. Analysis of the nucleotide sequence of Nms-25 revealed that this gene shows all the characteristics of an interrupted plant gene consisting of 13 exons and 12 introns. The promoter region of Nms-25 contains the common promoter elements of plant genes as well as motifs which are supposed to be involved in nodule-specific expression. There are two exon-like sequences in the gene named PE1 and PE2 which are not present in the cDNA clones of Medicago sativa cultivar Cardinal. Intron 9 carries a retrotransposon-like element, Tms1, which might be responsible for downstream deletion events in which a heptanucleotide, ATTAGCT, might have been involved. Most of the exons, exept 1, 12 and 13, are similar to each other both in length (54 bp) and sequence (up to 94% sequence similarity). All exons are interrupted by introns in the same phase (type I). It is suggested that exon-shuffling based on illegitimate recombination in which the ATTAGCT motif might have played an active role, and retrotransposon-mediated DNA rearrangements were the primary events in the molecular evolution of the Nms-25 gene. The nucleotide sequence of the genomic clone of Nms-25 from Medicago sativa is available from the EMBL/GenBank/DDBJ Nucleotide Sequence Databases under the accession number X13287.

Original languageEnglish
Pages (from-to)295-306
Number of pages12
JournalPlant Molecular Biology
Volume15
Issue number2
DOIs
Publication statusPublished - Aug 1990

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Retroelements
Medicago sativa
Gene Rearrangement
retrotransposons
exons
Exons
Nucleotides
Genes
nucleotide sequences
DNA
Introns
introns
Plant Genes
Nucleic Acid Databases
genes
Clone Cells
promoter regions
clones
Molecular Evolution
plant characteristics

Keywords

  • evolution by exon-shuffling
  • Medicago sativa (alfalfa)
  • Nms-25 (nodulin-25) DNA sequence
  • nodule-specific gene
  • retrotransposon
  • symbiotic nitrogen fixation

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

The nucleotide sequence of a nodule-specific gene, Nms-25 of Medicago sativa : its primary evolution via exon-shuffling and retrotransposon-mediated DNA rearrangements. / Végh, Zoltán; Vincze, Éva; Kadirov, Rafael; Tóth, Gábor; Kiss, G.

In: Plant Molecular Biology, Vol. 15, No. 2, 08.1990, p. 295-306.

Research output: Contribution to journalArticle

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abstract = "We present the primary structure of a nudule-specific gene, Nms-25 from Medicago sativa L. cultivar Nagysz{\'e}n{\'a}si. Analysis of the nucleotide sequence of Nms-25 revealed that this gene shows all the characteristics of an interrupted plant gene consisting of 13 exons and 12 introns. The promoter region of Nms-25 contains the common promoter elements of plant genes as well as motifs which are supposed to be involved in nodule-specific expression. There are two exon-like sequences in the gene named PE1 and PE2 which are not present in the cDNA clones of Medicago sativa cultivar Cardinal. Intron 9 carries a retrotransposon-like element, Tms1, which might be responsible for downstream deletion events in which a heptanucleotide, ATTAGCT, might have been involved. Most of the exons, exept 1, 12 and 13, are similar to each other both in length (54 bp) and sequence (up to 94{\%} sequence similarity). All exons are interrupted by introns in the same phase (type I). It is suggested that exon-shuffling based on illegitimate recombination in which the ATTAGCT motif might have played an active role, and retrotransposon-mediated DNA rearrangements were the primary events in the molecular evolution of the Nms-25 gene. The nucleotide sequence of the genomic clone of Nms-25 from Medicago sativa is available from the EMBL/GenBank/DDBJ Nucleotide Sequence Databases under the accession number X13287.",
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AB - We present the primary structure of a nudule-specific gene, Nms-25 from Medicago sativa L. cultivar Nagyszénási. Analysis of the nucleotide sequence of Nms-25 revealed that this gene shows all the characteristics of an interrupted plant gene consisting of 13 exons and 12 introns. The promoter region of Nms-25 contains the common promoter elements of plant genes as well as motifs which are supposed to be involved in nodule-specific expression. There are two exon-like sequences in the gene named PE1 and PE2 which are not present in the cDNA clones of Medicago sativa cultivar Cardinal. Intron 9 carries a retrotransposon-like element, Tms1, which might be responsible for downstream deletion events in which a heptanucleotide, ATTAGCT, might have been involved. Most of the exons, exept 1, 12 and 13, are similar to each other both in length (54 bp) and sequence (up to 94% sequence similarity). All exons are interrupted by introns in the same phase (type I). It is suggested that exon-shuffling based on illegitimate recombination in which the ATTAGCT motif might have played an active role, and retrotransposon-mediated DNA rearrangements were the primary events in the molecular evolution of the Nms-25 gene. The nucleotide sequence of the genomic clone of Nms-25 from Medicago sativa is available from the EMBL/GenBank/DDBJ Nucleotide Sequence Databases under the accession number X13287.

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