The No-go decay system degrades plant mRNAs that contain a long A-stretch in the coding region

István Szádeczky-Kardoss, Luca Gál, Andor Auber, János Taller, Dániel Silhavy

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

RNA quality control systems identify and degrade aberrant mRNAs, thereby preventing the accumulation of faulty proteins. Non-stop decay (NSD) and No-go decay (NGD) are closely related RNA quality control systems that act during translation. NSD degrades mRNAs lacking a stop codon, while NGD recognizes and decays mRNAs that contain translation elongation inhibitory structures. NGD has been intensively studied in yeast and animals but it has not been described in plants yet. In yeast, NGD is induced if the elongating ribosome is stalled by a strong inhibitory structure. Then, the mRNA is cleaved by an unknown nuclease and the cleavage fragments are degraded. Here we show that NGD also operates in plant. We tested several potential NGD cis-elements and found that in plants, unlike in yeast, only long A-stretches induce NGD. These long A-stretches trigger endonucleolytic cleavage, and then the 5′ fragments are degraded in a Pelota-, HBS1- and SKI2- dependent manner, while XRN4 eliminates the 3′ fragment. We also show that plant NGD operates gradually, the longer the A-stretch, the more efficient the cleavage. Our data suggest that mechanistically NGD is conserved in eukaryotes, although the NGD inducing cis-elements could be different. Moreover, we found that Arabidopsis AtPelota1 functions in both NGD and NSD, while AtPelota2 represses these quality control systems. The function of plant NGD will be discussed.

Original languageEnglish
Pages (from-to)19-27
Number of pages9
JournalPlant Science
Volume275
DOIs
Publication statusPublished - Oct 2018

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Keywords

  • Long A-stretch
  • No-go decay
  • Pelota2 dominant-negative mutant
  • Prevention of silencing amplification
  • Ribosome stalling

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

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