The nitric oxide pathway is amplified in venular vs arteriolar cultured rat mesenteric endothelial cells

L. Wágner, John G. Hoey, Aaron Erdely, Matthew A. Boegehold, Chris Baylis

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

To determine if there are differences in nitric oxide activity between pre- and postcapillary microvessels, we studied cultured rat mesenteric arteriolar and venular endothelial cells (RMAEC, RMVEC). We measured expression of endothelial nitric oxide synthase (eNOS), the activity of eNOS, and L-arginine transport in live RMAEC and RMVEC and the L-arginine content of RMAEC and RMVEC lysates. The abundance of eNOS was significantly greater in RMVEC vs RMAEC; this was also true for freshly harvested, pooled microvessels. Baseline NOS activity was higher in RMVEC than in RMAEC. NG-Monomethyl-L-arginine (L-NMA; 5 mM) inhibited NOS activity by -70-80% in both RMAEC and RMVEC, indicating that metabolism of L-arginine is largely via NOS. Intracellular L-arginine levels were higher in RMVEC vs RMAEC and well above the eNOS Km in both cell types. L-Arginine levels increased with L-NMA in both RMAEC and RMVEC, presumably due to reduced substrate utilization. Since L-arginine transport was not higher in RMVEC vs RMAEC, this may reflect higher intracellular arginine synthesis. A higher intrinsic level of baseline NO production in the postcapillary microvascular endothelium may reflect both the contribution of venular derived NO to control of arteriolar tone and a key role of venular-derived NO in local thrombosis control.

Original languageEnglish
Pages (from-to)401-409
Number of pages9
JournalMicrovascular Research
Volume62
Issue number3
DOIs
Publication statusPublished - 2001

Fingerprint

Endothelial cells
Arginine
Rats
Nitric Oxide
Endothelial Cells
Nitric Oxide Synthase Type III
Microvessels
omega-N-Methylarginine
Metabolism
Endothelium
Thrombosis
Substrates

Keywords

  • L-arginine
  • L-NMA
  • Microcirculation
  • Nitric oxide synthase

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine

Cite this

The nitric oxide pathway is amplified in venular vs arteriolar cultured rat mesenteric endothelial cells. / Wágner, L.; Hoey, John G.; Erdely, Aaron; Boegehold, Matthew A.; Baylis, Chris.

In: Microvascular Research, Vol. 62, No. 3, 2001, p. 401-409.

Research output: Contribution to journalArticle

Wágner, L. ; Hoey, John G. ; Erdely, Aaron ; Boegehold, Matthew A. ; Baylis, Chris. / The nitric oxide pathway is amplified in venular vs arteriolar cultured rat mesenteric endothelial cells. In: Microvascular Research. 2001 ; Vol. 62, No. 3. pp. 401-409.
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