The Nimrod transmembrane receptor Eater is required for hemocyte attachment to the sessile compartment in Drosophila melanogaster

Andrew J. Bretscher, Viktor Honti, Olivier Binggeli, Olivier Burri, Mickael Poidevin, E. Kurucz, János Zsámboki, I. Andó, Bruno Lemaitre

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Eater is an EGF-like repeat transmembrane receptor of the Nimrod family and is expressed in Drosophila hemocytes. Eater was initially identified for its role in phagocytosis of both Gram-positive and Gram-negative bacteria. We have deleted eater and show that it appears to be required for efficient phagocytosis of Gram-positive but not Gram-negative bacteria. However, the most striking phenotype of eater deficient larvae is the near absence of sessile hemocytes, both plasmatocyte and crystal cell types. The eater deletion is the first loss of function mutation identified that causes absence of the sessile hemocyte state. Our study shows that Eater is required cell-autonomously in plasmatocytes for sessility. However, the presence of crystal cells in the sessile compartment requires Eater in plasmatocytes. We also show that eater deficient hemocytes exhibit a cell adhesion defect. Collectively, our data uncovers a new requirement of Eater in enabling hemocyte attachment at the sessile compartment and points to a possible role of Nimrod family members in hemocyte adhesion.

Original languageEnglish
Pages (from-to)355-363
Number of pages9
JournalBiology Open
Volume4
Issue number3
DOIs
Publication statusPublished - Mar 15 2015

Fingerprint

Hemocytes
hemocytes
Drosophila melanogaster
Bacteria
Crystals
receptors
Cell adhesion
Epidermal Growth Factor
Adhesion
Gram-Negative Bacteria
phagocytosis
Phagocytosis
Gram-negative bacteria
Defects
crystals
family relations
cells
cell adhesion
Cell Adhesion
Drosophila

Keywords

  • Eater
  • EGF-like
  • Hemocyte
  • Receptor
  • Sessile

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

The Nimrod transmembrane receptor Eater is required for hemocyte attachment to the sessile compartment in Drosophila melanogaster. / Bretscher, Andrew J.; Honti, Viktor; Binggeli, Olivier; Burri, Olivier; Poidevin, Mickael; Kurucz, E.; Zsámboki, János; Andó, I.; Lemaitre, Bruno.

In: Biology Open, Vol. 4, No. 3, 15.03.2015, p. 355-363.

Research output: Contribution to journalArticle

Bretscher, Andrew J. ; Honti, Viktor ; Binggeli, Olivier ; Burri, Olivier ; Poidevin, Mickael ; Kurucz, E. ; Zsámboki, János ; Andó, I. ; Lemaitre, Bruno. / The Nimrod transmembrane receptor Eater is required for hemocyte attachment to the sessile compartment in Drosophila melanogaster. In: Biology Open. 2015 ; Vol. 4, No. 3. pp. 355-363.
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