The neuropeptide FMRFamide can protect cells against apoptosis in the snail digestive gland

T. Roszer, J. Kappelmayer, G. G. Nagy, A. J. Szentmiklósi, A. G. Basnakian, G. Bánfalvi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

FMRFamide-related peptides are widespread neurotransmitters or neurohormones regulating somatic or visceral motor activity. Some recent data indicate that these neuropeptides may be involved in the control of cell proliferation and apoptosis. In this work we investigated the possible effect of FMRFamide on cell viability in an invertebrate-type proliferating tissue. As a model, we used the midintestinal gland of the snail, Helix lucorum Linnaeus. Immunohistochemistry demonstrated the direct innervation of the gland cells by FMRFamide-containing nerve fibers. Midintestinal glands of snails were injected with 50 μM FMRFamide and the control with sterile deionised water or bovine serum albumin (BSA). Injections were administrated 4 times. Transmission electron microscopy, annexin V-labeling, thiazolyl blue (MTT) viability tests and ploidy analyses were carried out to define the viable/dead cell ratio in the tissue samples. FMRFamide increased the MTT-reduction of tissues, reduced the amount of apoptotic nuclei and annexin V-labeled cells. Deionised water or BSA injection induced cell death. Cell cyle analysis revealed that FMRFamide significantly elevated the amount of cells in G0/G1 phase, but did not induce mitosis. We conclude, that the FMRFamide can be a life-signal for cells, protect them from apoptosis without altering mitosis.

Original languageEnglish
Pages (from-to)173-182
Number of pages10
JournalApoptosis
Volume11
Issue number2
DOIs
Publication statusPublished - Feb 2006

Fingerprint

FMRFamide
Snails
Neuropeptides
Apoptosis
Deionized water
Annexin A5
Tissue
Bovine Serum Albumin
Mitosis
Neurotransmitter Agents
Helix (Snails)
Cell Cycle Resting Phase
Injections
Water
Ploidies
Cell proliferation
G1 Phase
Cell death
Invertebrates
Transmission Electron Microscopy

Keywords

  • Cell cycle
  • Cell death
  • FMRFamide
  • Gastropods
  • Neuropeptides

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

Cite this

The neuropeptide FMRFamide can protect cells against apoptosis in the snail digestive gland. / Roszer, T.; Kappelmayer, J.; Nagy, G. G.; Szentmiklósi, A. J.; Basnakian, A. G.; Bánfalvi, G.

In: Apoptosis, Vol. 11, No. 2, 02.2006, p. 173-182.

Research output: Contribution to journalArticle

Roszer, T. ; Kappelmayer, J. ; Nagy, G. G. ; Szentmiklósi, A. J. ; Basnakian, A. G. ; Bánfalvi, G. / The neuropeptide FMRFamide can protect cells against apoptosis in the snail digestive gland. In: Apoptosis. 2006 ; Vol. 11, No. 2. pp. 173-182.
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