The negative surface charge density of plasmalemma vesicles from wheat and oat roots

Ian M. Møller, Tomas Lundborg, A. Bérczi

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Plasmalemma vesicles were isolated in a sucrose-containing medium from wheat and oat roots and the net negative surface charge density was determined with 9-aminoacridine fluorescence [Chow, W.S. and Barber, J. (1980) J. Biochem. Biophys. Methods 3, 173-185]. The outer surface of the vesicles (measured in the presence of sucrose) had densities of - 16 to - 20 mC·m-2 and -29 mC·m-2 for wheat and oat roots, respectively. The inner surface - presumed to be the cytoplasmic side and calculated from the values measured in the presence and absence of sucrose - was more negative, and its size depended on the salt status of the roots.

Original languageEnglish
Pages (from-to)181-185
Number of pages5
JournalFEBS Letters
Volume167
Issue number1
DOIs
Publication statusPublished - Feb 13 1984

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Surface charge
Charge density
Triticum
Sucrose
Aminacrine
Salts
Fluorescence
Avena

Keywords

  • 9-Aminoacridine
  • Membrane asymmetry
  • Plasmalemma
  • Surface charge density
  • Surface potential

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The negative surface charge density of plasmalemma vesicles from wheat and oat roots. / Møller, Ian M.; Lundborg, Tomas; Bérczi, A.

In: FEBS Letters, Vol. 167, No. 1, 13.02.1984, p. 181-185.

Research output: Contribution to journalArticle

Møller, Ian M. ; Lundborg, Tomas ; Bérczi, A. / The negative surface charge density of plasmalemma vesicles from wheat and oat roots. In: FEBS Letters. 1984 ; Vol. 167, No. 1. pp. 181-185.
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