The N-Terminal Intrinsically Disordered Domain of Mgm101p Is Localized to the Mitochondrial Nucleoid

David C. Hayward, Z. Dosztányi, George Desmond Clark-Walker

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The mitochondrial genome maintenance gene, MGM101, is essential for yeasts that depend on mitochondrial DNA replication. Previously, in Saccharomyces cerevisiae, it has been found that the carboxy-terminal two-thirds of Mgm101p has a functional core. Furthermore, there is a high level of amino acid sequence conservation in this region from widely diverse species. By contrast, the amino-terminal region, that is also essential for function, does not have recognizable conservation. Using a bioinformatic approach we find that the functional core from yeast and a corresponding region of Mgm101p from the coral Acropora millepora have an ordered structure, while the N-terminal domains of sequences from yeast and coral are predicted to be disordered. To examine whether ordered and disordered domains of Mgm101p have specific or general functions we made chimeric proteins from yeast and coral by swapping the two regions. We find, by an in vivo assay in S.cerevisiae, that the ordered domain of A.millepora can functionally replace the yeast core region but the disordered domain of the coral protein cannot substitute for its yeast counterpart. Mgm101p is found in the mitochondrial nucleoid along with enzymes and proteins involved in mtDNA replication. By attaching green fluorescent protein to the N-terminal disordered domain of yeast Mgm101p we find that GFP is still directed to the mitochondrial nucleoid where full-length Mgm101p-GFP is targeted.

Original languageEnglish
Article numbere56465
JournalPLoS One
Volume8
Issue number2
DOIs
Publication statusPublished - Feb 13 2013

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Yeast
Anthozoa
Yeasts
yeasts
corals
Mitochondrial DNA
Saccharomyces cerevisiae
Conservation
mitochondrial DNA
Genes
Acropora millepora
recombinant fusion proteins
Fungal Proteins
Mitochondrial Genome
Green Fluorescent Proteins
Computational Biology
DNA Replication
DNA replication
Bioinformatics
Amino Acid Sequence

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

The N-Terminal Intrinsically Disordered Domain of Mgm101p Is Localized to the Mitochondrial Nucleoid. / Hayward, David C.; Dosztányi, Z.; Clark-Walker, George Desmond.

In: PLoS One, Vol. 8, No. 2, e56465, 13.02.2013.

Research output: Contribution to journalArticle

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