The N-terminal 303 amino acids of the human cytomegalovirus envelope glycoprotein B (UL55) and the exon 4 region of the major immediate early protein 1 (UL123) induce a cytotoxic T-cell response

Klara Berencsi, Eva Gönczöl, Valeria Endresz, John Kough, Shin Takeda, Zsofia Gyulay, Stanley A. Plotkin, Robert F. Rando

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We reported earlier that an adenovirus (Ad) recombinant expressing the full-length human cytomegalovirus (HCMV) glycoprotein B (gB) gene induces gB-specific cytotoxic T lymphocyte (CTL) responses in CBA (H-2(k)) mice (Berencsi et al., J. Gen. Virol. 74, 257-2512, 1993). Here we show that mice immunized with Ad recombinant viruses expressing truncated forms of the gB gene containing the first 700 (Ad-700), 465 (Ad-465) or 303 (Ad-303) amino acids of gB or an Ad construct containing exon 4 (E4) of the HCMV immediate early 1 (IE1) gene (Ad-IE1 (E4)) demonstrate HCMV-specific CTL 1 responses. These data suggest the importance of the first 303 amino acids of the gB polypeptide and the IE1 E4 product in designing a vaccine to induce anti-HCMV CTL responses.

Original languageEnglish
Pages (from-to)369-374
Number of pages6
JournalVaccine
Volume14
Issue number5
DOIs
Publication statusPublished - Apr 1996

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Keywords

  • Ad-HCMV recombinants
  • CTL epitope
  • HCMV
  • IE1 exon 4

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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