The N-acetylglucosamine catabolic gene cluster in Trichoderma reesei is controlled by the Ndt80-like transcription factor RON1

Lisa Kappel, Romana Gaderer, Michel Flipphi, Verena Seidl-Seiboth

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Chitin is an important structural constituent of fungal cell walls composed of N-acetylglucosamine (GlcNAc) monosaccharides, but catabolism of GlcNAc has not been studied in filamentous fungi so far. In the yeast Candida albicans, the genes encoding the three enzymes responsible for stepwise conversion of GlcNAc to fructose-6-phosphate are clustered. In this work, we analysed GlcNAc catabolism in ascomycete filamentous fungi and found that the respective genes are also clustered in these fungi. In contrast to C.albicans, the cluster often contains a gene for an Ndt80-like transcription factor, which we named RON1 (regulator of N-acetylglucosamine catabolism 1). Further, a gene for a glycoside hydrolase 3 protein related to bacterial N-acetylglucosaminidases can be found in the GlcNAc gene cluster in filamentous fungi. Functional analysis in Trichoderma reesei showed that the transcription factor RON1 is a key activator of the GlcNAc gene cluster and essential for GlcNAc catabolism. Furthermore, we present an evolutionary analysis of Ndt80-like proteins in Ascomycota. All GlcNAc cluster genes, as well as the GlcNAc transporter gene ngt1, and an additional transcriptional regulator gene, csp2, encoding the homolog of Neurospora crassaCSP2/GRHL, were functionally characterised by gene expression analysis and phenotypic characterisation of knockout strains in T.reesei.

Original languageEnglish
Pages (from-to)640-657
Number of pages18
JournalMolecular Microbiology
Volume99
Issue number4
DOIs
Publication statusPublished - Feb 1 2016

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Trichoderma
Acetylglucosamine
Multigene Family
Transcription Factors
Fungi
Ascomycota
Genes
Neurospora
Acetylglucosaminidase
Chitin
Monosaccharides
Glycoside Hydrolases
Regulator Genes
Candida albicans
Cell Wall
Proteins
Yeasts
Gene Expression
Enzymes

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

The N-acetylglucosamine catabolic gene cluster in Trichoderma reesei is controlled by the Ndt80-like transcription factor RON1. / Kappel, Lisa; Gaderer, Romana; Flipphi, Michel; Seidl-Seiboth, Verena.

In: Molecular Microbiology, Vol. 99, No. 4, 01.02.2016, p. 640-657.

Research output: Contribution to journalArticle

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