The mutualistic fungus piriformospora indica protects barley roots from a loss of antioxidant capacity caused by the necrotrophic pathogen fusarium culmorum

Borbála D. Harrach, Helmut Baltruschat, B. Barna, J. Fodor, Karl Heinz Kogel

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Fusarium culmorum causes root rot in barley (Hordeum vulgare), resulting in severely reduced plant growth and yield. Pretreatment of roots with chlamydospores of the mutualistic root-colonizing basidiomycete Piriformospora indica (subdivision Agaricomycotina) prevented necrotization of root tissues and plant growth retardation commonly associated with Fusarium root rot. Quantification of Fusarium infections with a real-time polymerase chain reaction assay revealed a correlation between root rot symptoms and the relative amount of fungal DNA. Fusarium-infected roots showed reduced levels of ascorbate and glutathione (GSH), along with reduced activities of antioxidant enzymes such as superoxide dismutase, ascorbate peroxidase, GSH reductase, dehydroascorbate reductase, and monodehydroascorbate reductase. Consistent with this, Fusariuminfected roots showed elevated levels of lipid hydroperoxides and decreased ratios of reduced to oxidized forms of ascorbate and GSH. In clear contrast, roots treated with P. indica prior to inoculation with F. culmorum showed levels of ascorbate and GSH that were similar to controls. Likewise, lipid peroxidation and the overall reduction in antioxidant enzyme activities were largely attenuated by P. indica in roots challenged by F. culmorum. These results suggest that P. indica protects roots from necrotrophic pathogens, at least partly, through activating the plant's antioxidant capacity.

Original languageEnglish
Pages (from-to)599-605
Number of pages7
JournalMolecular Plant-Microbe Interactions
Volume26
Issue number5
DOIs
Publication statusPublished - May 2013

Fingerprint

Fusarium culmorum
Fusarium
Hordeum
Fungi
root rot
Antioxidants
barley
antioxidants
glutathione dehydrogenase (ascorbate)
fungi
pathogens
Oxidoreductases
Fungal DNA
Fusariosis
Ascorbate Peroxidases
Basidiomycota
Agaricomycotina
Plant Roots
Lipid Peroxides
plant growth

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

Cite this

The mutualistic fungus piriformospora indica protects barley roots from a loss of antioxidant capacity caused by the necrotrophic pathogen fusarium culmorum. / Harrach, Borbála D.; Baltruschat, Helmut; Barna, B.; Fodor, J.; Kogel, Karl Heinz.

In: Molecular Plant-Microbe Interactions, Vol. 26, No. 5, 05.2013, p. 599-605.

Research output: Contribution to journalArticle

@article{6271d17818c14337aa07751f4adb1896,
title = "The mutualistic fungus piriformospora indica protects barley roots from a loss of antioxidant capacity caused by the necrotrophic pathogen fusarium culmorum",
abstract = "Fusarium culmorum causes root rot in barley (Hordeum vulgare), resulting in severely reduced plant growth and yield. Pretreatment of roots with chlamydospores of the mutualistic root-colonizing basidiomycete Piriformospora indica (subdivision Agaricomycotina) prevented necrotization of root tissues and plant growth retardation commonly associated with Fusarium root rot. Quantification of Fusarium infections with a real-time polymerase chain reaction assay revealed a correlation between root rot symptoms and the relative amount of fungal DNA. Fusarium-infected roots showed reduced levels of ascorbate and glutathione (GSH), along with reduced activities of antioxidant enzymes such as superoxide dismutase, ascorbate peroxidase, GSH reductase, dehydroascorbate reductase, and monodehydroascorbate reductase. Consistent with this, Fusariuminfected roots showed elevated levels of lipid hydroperoxides and decreased ratios of reduced to oxidized forms of ascorbate and GSH. In clear contrast, roots treated with P. indica prior to inoculation with F. culmorum showed levels of ascorbate and GSH that were similar to controls. Likewise, lipid peroxidation and the overall reduction in antioxidant enzyme activities were largely attenuated by P. indica in roots challenged by F. culmorum. These results suggest that P. indica protects roots from necrotrophic pathogens, at least partly, through activating the plant's antioxidant capacity.",
author = "Harrach, {Borb{\'a}la D.} and Helmut Baltruschat and B. Barna and J. Fodor and Kogel, {Karl Heinz}",
year = "2013",
month = "5",
doi = "10.1094/MPMI-09-12-0216-R",
language = "English",
volume = "26",
pages = "599--605",
journal = "Molecular Plant-Microbe Interactions",
issn = "0894-0282",
publisher = "American Phytopathological Society",
number = "5",

}

TY - JOUR

T1 - The mutualistic fungus piriformospora indica protects barley roots from a loss of antioxidant capacity caused by the necrotrophic pathogen fusarium culmorum

AU - Harrach, Borbála D.

AU - Baltruschat, Helmut

AU - Barna, B.

AU - Fodor, J.

AU - Kogel, Karl Heinz

PY - 2013/5

Y1 - 2013/5

N2 - Fusarium culmorum causes root rot in barley (Hordeum vulgare), resulting in severely reduced plant growth and yield. Pretreatment of roots with chlamydospores of the mutualistic root-colonizing basidiomycete Piriformospora indica (subdivision Agaricomycotina) prevented necrotization of root tissues and plant growth retardation commonly associated with Fusarium root rot. Quantification of Fusarium infections with a real-time polymerase chain reaction assay revealed a correlation between root rot symptoms and the relative amount of fungal DNA. Fusarium-infected roots showed reduced levels of ascorbate and glutathione (GSH), along with reduced activities of antioxidant enzymes such as superoxide dismutase, ascorbate peroxidase, GSH reductase, dehydroascorbate reductase, and monodehydroascorbate reductase. Consistent with this, Fusariuminfected roots showed elevated levels of lipid hydroperoxides and decreased ratios of reduced to oxidized forms of ascorbate and GSH. In clear contrast, roots treated with P. indica prior to inoculation with F. culmorum showed levels of ascorbate and GSH that were similar to controls. Likewise, lipid peroxidation and the overall reduction in antioxidant enzyme activities were largely attenuated by P. indica in roots challenged by F. culmorum. These results suggest that P. indica protects roots from necrotrophic pathogens, at least partly, through activating the plant's antioxidant capacity.

AB - Fusarium culmorum causes root rot in barley (Hordeum vulgare), resulting in severely reduced plant growth and yield. Pretreatment of roots with chlamydospores of the mutualistic root-colonizing basidiomycete Piriformospora indica (subdivision Agaricomycotina) prevented necrotization of root tissues and plant growth retardation commonly associated with Fusarium root rot. Quantification of Fusarium infections with a real-time polymerase chain reaction assay revealed a correlation between root rot symptoms and the relative amount of fungal DNA. Fusarium-infected roots showed reduced levels of ascorbate and glutathione (GSH), along with reduced activities of antioxidant enzymes such as superoxide dismutase, ascorbate peroxidase, GSH reductase, dehydroascorbate reductase, and monodehydroascorbate reductase. Consistent with this, Fusariuminfected roots showed elevated levels of lipid hydroperoxides and decreased ratios of reduced to oxidized forms of ascorbate and GSH. In clear contrast, roots treated with P. indica prior to inoculation with F. culmorum showed levels of ascorbate and GSH that were similar to controls. Likewise, lipid peroxidation and the overall reduction in antioxidant enzyme activities were largely attenuated by P. indica in roots challenged by F. culmorum. These results suggest that P. indica protects roots from necrotrophic pathogens, at least partly, through activating the plant's antioxidant capacity.

UR - http://www.scopus.com/inward/record.url?scp=84876008947&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84876008947&partnerID=8YFLogxK

U2 - 10.1094/MPMI-09-12-0216-R

DO - 10.1094/MPMI-09-12-0216-R

M3 - Article

C2 - 23405867

AN - SCOPUS:84876008947

VL - 26

SP - 599

EP - 605

JO - Molecular Plant-Microbe Interactions

JF - Molecular Plant-Microbe Interactions

SN - 0894-0282

IS - 5

ER -