The morphology of microtubules in incubated synaptosomes. Effect of low temperature and vinblastine

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Abstract

A time-dependent rise in the percentage of microtubule-containing synaptosomes was observed reaching a 41-47% peak after 30 min incubation at room temperature (22-25 ° C) in a saline medium. Fixation at low temperature or incubation with vinblastine decreased markedly the number of synaptosomes containing microtubules. It was concluded that incubation with cations and processing at room temperature were prerequisites of optimal preservation of synaptosomal microtubules. Their spatial orientation relative to the synaptic cleft was deduced from statistical examination of profiles in different orientations.

Original languageEnglish
Pages (from-to)387-393
Number of pages7
JournalExperimental Brain Research
Volume35
Issue number2
DOIs
Publication statusPublished - Apr 1979

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Vinblastine
Synaptosomes
Microtubules
Temperature
Cations

Keywords

  • Microtubules
  • Synaptosome

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The morphology of microtubules in incubated synaptosomes. Effect of low temperature and vinblastine. / Hajós, F.; Csillág, A.; Kálmán, M.

In: Experimental Brain Research, Vol. 35, No. 2, 04.1979, p. 387-393.

Research output: Contribution to journalArticle

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