The monoterpenoid (s)-carvone massively up-regulates several classes of glutathione s-transferase genes in tobacco leaf discs

C. Juhász, G. Gullner

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The monoterpenoid (S)-carvone was shown earlier to induce glutathione S-transferase (GST) activity and to attenuate necrotic lesion formation in Tobacco mosaic virus (TMV) inoculated, hypersensitively reacting tobacco Xanthi-nc plants. To explore structure-activity relationships, three monoterpenes and five monoterpenoids were tested for their ability to induce increased GST activity in tobacco leaf discs. (S)-Carvone proved to be the most potent inducer. (S)-Carvone treatments markedly up-regulated the expression of several tobacco GST genes belonging to the phi, tau and theta classes, particularly in the case of tau class GSTs. Furthermore, TMV inoculation slightly induced the transcript abundance of the GSTT2 gene. The expression of three catalase (CAT) genes was also examined in (S)-carvone-treated tobacco leaf discs. The expression of CAT1 was not influenced by (S)-carvone treatments, whereas that of CAT2 was significantly repressed. (S)-Carvone very weakly induced the transcription of the CAT3 gene. The effect of the opposite enantiomer, (R)-carvone on the expression of GST and CAT genes was similar to that of (S)-carvone. Our results suggest that the antioxidative reactions catalyzed by tau and theta class GSTs contribute to the attenuation of necrotic disease symptoms in TMV-inoculated tobacco plants.

Original languageEnglish
Pages (from-to)163-176
Number of pages14
JournalActa Phytopathologica et Entomologica Hungarica
Volume49
Issue number2
DOIs
Publication statusPublished - Dec 1 2014

Fingerprint

carvone
transferases
monoterpenoids
glutathione
tobacco
glutathione transferase
Tobacco mosaic virus
leaves
genes
catalase
lesions (plant)
enantiomers
structure-activity relationships
signs and symptoms (plants)
transcription (genetics)

Keywords

  • (S)-carvone
  • Catalase
  • Glutathione S-transferase
  • Monoterpene
  • Monoterpenoid
  • Necrotic lesion
  • Tobacco
  • Tobacco mosaic virus

ASJC Scopus subject areas

  • Insect Science
  • Plant Science

Cite this

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abstract = "The monoterpenoid (S)-carvone was shown earlier to induce glutathione S-transferase (GST) activity and to attenuate necrotic lesion formation in Tobacco mosaic virus (TMV) inoculated, hypersensitively reacting tobacco Xanthi-nc plants. To explore structure-activity relationships, three monoterpenes and five monoterpenoids were tested for their ability to induce increased GST activity in tobacco leaf discs. (S)-Carvone proved to be the most potent inducer. (S)-Carvone treatments markedly up-regulated the expression of several tobacco GST genes belonging to the phi, tau and theta classes, particularly in the case of tau class GSTs. Furthermore, TMV inoculation slightly induced the transcript abundance of the GSTT2 gene. The expression of three catalase (CAT) genes was also examined in (S)-carvone-treated tobacco leaf discs. The expression of CAT1 was not influenced by (S)-carvone treatments, whereas that of CAT2 was significantly repressed. (S)-Carvone very weakly induced the transcription of the CAT3 gene. The effect of the opposite enantiomer, (R)-carvone on the expression of GST and CAT genes was similar to that of (S)-carvone. Our results suggest that the antioxidative reactions catalyzed by tau and theta class GSTs contribute to the attenuation of necrotic disease symptoms in TMV-inoculated tobacco plants.",
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