The mode of action of primycin

I. Horváth, M. Kramer, P. Bauer, K. G. Büki

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Primycin, an antibiotic active against Gram-positive microorganisms increased the permeability of Bacillus subtilis cell membranes when used in bacteriostatic concentrations. On addition of the antibiotic to the washed cell suspension, a dose-dependent increase in the conductivity was observed. Furthermore, an enhanced leakage of the nucleotides (measured by the32P-ATP release from the32P-labelled culture) could be detected. To get more information about the mechanism of the primycin-membrane interaction, the effect of the antibiotic on the ATPase activity of membrane vesicles prepared from both Bacillus subtilis and Escherichia coli B was studied. Activation was found at about 0.5 nmol antibiotic/μg protein and its extent was approximately the same as with sonicated membranes used as controls. Stimulation of ATPase activity was also achieved with vesicles prewashed with 3 mM Tris-HCl buffer. Purified membrane ATPase from Bacillus subtilis could not be activated by primycin at all; above 0.3 nmol/μg protein concentration the enzyme was inhibited. When acting on membrane vesicles isolated from Escherichia coli B, inhibition without previous activation was observed, although sonication caused a substantial activation on the ATPase of these membranes. These observations confirmed our suggestion that the primary target of primycin action is the cell membrane in Gram-positive microorganisms.

Original languageEnglish
Pages (from-to)135-139
Number of pages5
JournalArchives of Microbiology
Volume121
Issue number2
DOIs
Publication statusPublished - May 1979

Fingerprint

Membranes
Adenosine Triphosphatases
Bacilli
Bacillus subtilis
Anti-Bacterial Agents
Chemical activation
Cell membranes
Microorganisms
Escherichia coli
Cell Membrane
Tromethamine
Sonication
primycin
Permeability
Suspensions
Buffers
Proteins
Nucleotides
Adenosine Triphosphate
Enzymes

Keywords

  • Antibiotic
  • ATPase
  • Cell membrane
  • Primycin

ASJC Scopus subject areas

  • Microbiology

Cite this

Horváth, I., Kramer, M., Bauer, P., & Büki, K. G. (1979). The mode of action of primycin. Archives of Microbiology, 121(2), 135-139. https://doi.org/10.1007/BF00689977

The mode of action of primycin. / Horváth, I.; Kramer, M.; Bauer, P.; Büki, K. G.

In: Archives of Microbiology, Vol. 121, No. 2, 05.1979, p. 135-139.

Research output: Contribution to journalArticle

Horváth, I, Kramer, M, Bauer, P & Büki, KG 1979, 'The mode of action of primycin', Archives of Microbiology, vol. 121, no. 2, pp. 135-139. https://doi.org/10.1007/BF00689977
Horváth, I. ; Kramer, M. ; Bauer, P. ; Büki, K. G. / The mode of action of primycin. In: Archives of Microbiology. 1979 ; Vol. 121, No. 2. pp. 135-139.
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