A good correlation was found between the crystal formation capacity and biological effectiveness of the vinca alkaloids. Crystal formation probably occurs in two steps: (1) disruption of microtubules into soluble tubulin, and (2) irregular polymerization of tubulin into hexagonal crystals. The crystal formation induced by vinca alkaloids was influenced by colchicine, podophylline, SH inhibitors (N ethyl maleimide and thiomerosal) and phosphodiesterase inhibitors (theophylline and dibutyryl cAMP). Colchicine treatment enhanced the number of paracrystals. The SH inhibitors and phosphodiesterase inhibitors decreased the number of alkaloid induced crystals. In addition to the appearance of paracrystals, the alkaloids and alkylating agents induced the formation of numerous microfilaments.
|Number of pages||7|
|Publication status||Published - Jan 1 1976|
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