The Melting Diagram of Protein Solutions and Its Thermodynamic Interpretation

K. Tompa, M. Bokor, Péter Tompa

Research output: Contribution to journalArticle

Abstract

Here we present a novel method for the characterization of the hydration of protein solutions based on measuring and evaluating two-component wide-line ¹H NMR signals. We also provide a description of key elements of the procedure conceived for the thermodynamic interpretation of such results. These interdependent experimental and theoretical treatments provide direct experimental insight into the potential energy surface of proteins. The utility of our approach is demonstrated through the examples of two proteins of distinct structural classes: the globular, structured ubiquitin; and the intrinsically disordered ERD10 (early response to dehydration 10). We provide a detailed analysis and interpretation of data recorded earlier by cooling and slowly warming the protein solutions through thermal equilibrium states. We introduce and use order parameters that can be thus derived to characterize the distribution of potential energy barriers inhibiting the movement of water molecules bound to the surface of the protein. Our results enable a quantitative description of the ratio of ordered and disordered parts of proteins, and of the energy relations of protein⁻water bonds in aqueous solutions of the proteins.

Original languageEnglish
JournalInternational Journal of Molecular Sciences
Volume19
Issue number11
DOIs
Publication statusPublished - Nov 12 2018

Fingerprint

Thermodynamics
Freezing
Melting
diagrams
melting
proteins
Proteins
thermodynamics
Membrane Proteins
Water Movements
Potential energy surfaces
Energy barriers
potential energy
Ubiquitin
Potential energy
Dehydration
Hydration
Hot Temperature
Nuclear magnetic resonance
dehydration

Keywords

  • hydration
  • protein
  • wide-line 1H NMR

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

The Melting Diagram of Protein Solutions and Its Thermodynamic Interpretation. / Tompa, K.; Bokor, M.; Tompa, Péter.

In: International Journal of Molecular Sciences, Vol. 19, No. 11, 12.11.2018.

Research output: Contribution to journalArticle

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