The Medicago species A2-type cyclin is auxin regulated and involved in meristem formation but dispensable for endoreduplication-associated developmental programs

François Roudier, Elena Fedorova, Manuel Lebris, Phillippe Lecomte, J. Györgyey, Daniele Vaubert, G. Horváth, Pierre Abad, Adam Kondorosi, É. Kondorosi

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Phytohormones as well as temporal and spatial regulation of the cell cycle play a key role in plant development. Here, we investigated the function and regulation of an alfalfa (Medicago sativa) A2-type cyclin in three distinct root developmental programs: in primary and secondary root development, nodule development, and nematode-elicited gall formation. Using transgenic plants carrying the Medsa;cycA2;2 promoter-β-glucuronidase gene fusion, in combination with other techniques, cycA2;2 expression was localized in meristems and proliferating cells in the lateral root and nodule primordia. Rapid induction of cycA2;2 by Nod factors demonstrated that this gene is implicated in cell cycle activation of differentiated cells developing to nodule primordia. Surprisingly, cycA2;2 was repressed in the endoreduplicating, division-arrested cells both during nodule development and formation of giant cells in nematode-induced galls, indicating that CycA2;2 was dispensable for S-phase in endoreduplication cycles. Overexpression of cycA2;2 in transgenic plants corresponded to wild type protein levels and had no apparent phenotype. In contrast, antisense expression of cycA2;2 halted regeneration of somatic embryos, suggesting a role for CycA2;2 in the formation or activity of apical meristems. Expression of cycA2;2 was up-regulated by auxins, as expected from the presence of auxin response elements in the promoter. Moreover, auxin also affected the spatial expression pattern of this cyclin by shifting the cycA2;2 expression from the phloem to the xylem poles.

Original languageEnglish
Pages (from-to)1091-1103
Number of pages13
JournalPlant Physiology
Volume131
Issue number3
DOIs
Publication statusPublished - Mar 1 2003

Fingerprint

Cyclin A2
Endoreduplication
Medicago
endopolyploidy
Indoleacetic Acids
Meristem
cyclins
meristems
auxins
Medicago sativa
Genetically Modified Plants
galls
transgenic plants
cell cycle
Cell Cycle
promoter regions
Nematoda
Phloem
Xylem
Cyclins

ASJC Scopus subject areas

  • Plant Science

Cite this

The Medicago species A2-type cyclin is auxin regulated and involved in meristem formation but dispensable for endoreduplication-associated developmental programs. / Roudier, François; Fedorova, Elena; Lebris, Manuel; Lecomte, Phillippe; Györgyey, J.; Vaubert, Daniele; Horváth, G.; Abad, Pierre; Kondorosi, Adam; Kondorosi, É.

In: Plant Physiology, Vol. 131, No. 3, 01.03.2003, p. 1091-1103.

Research output: Contribution to journalArticle

Roudier, François ; Fedorova, Elena ; Lebris, Manuel ; Lecomte, Phillippe ; Györgyey, J. ; Vaubert, Daniele ; Horváth, G. ; Abad, Pierre ; Kondorosi, Adam ; Kondorosi, É. / The Medicago species A2-type cyclin is auxin regulated and involved in meristem formation but dispensable for endoreduplication-associated developmental programs. In: Plant Physiology. 2003 ; Vol. 131, No. 3. pp. 1091-1103.
@article{1138e68aeb1045d68e16ce3931e99d45,
title = "The Medicago species A2-type cyclin is auxin regulated and involved in meristem formation but dispensable for endoreduplication-associated developmental programs",
abstract = "Phytohormones as well as temporal and spatial regulation of the cell cycle play a key role in plant development. Here, we investigated the function and regulation of an alfalfa (Medicago sativa) A2-type cyclin in three distinct root developmental programs: in primary and secondary root development, nodule development, and nematode-elicited gall formation. Using transgenic plants carrying the Medsa;cycA2;2 promoter-β-glucuronidase gene fusion, in combination with other techniques, cycA2;2 expression was localized in meristems and proliferating cells in the lateral root and nodule primordia. Rapid induction of cycA2;2 by Nod factors demonstrated that this gene is implicated in cell cycle activation of differentiated cells developing to nodule primordia. Surprisingly, cycA2;2 was repressed in the endoreduplicating, division-arrested cells both during nodule development and formation of giant cells in nematode-induced galls, indicating that CycA2;2 was dispensable for S-phase in endoreduplication cycles. Overexpression of cycA2;2 in transgenic plants corresponded to wild type protein levels and had no apparent phenotype. In contrast, antisense expression of cycA2;2 halted regeneration of somatic embryos, suggesting a role for CycA2;2 in the formation or activity of apical meristems. Expression of cycA2;2 was up-regulated by auxins, as expected from the presence of auxin response elements in the promoter. Moreover, auxin also affected the spatial expression pattern of this cyclin by shifting the cycA2;2 expression from the phloem to the xylem poles.",
author = "Fran{\cc}ois Roudier and Elena Fedorova and Manuel Lebris and Phillippe Lecomte and J. Gy{\"o}rgyey and Daniele Vaubert and G. Horv{\'a}th and Pierre Abad and Adam Kondorosi and {\'E}. Kondorosi",
year = "2003",
month = "3",
day = "1",
doi = "10.1104/pp.102.011122",
language = "English",
volume = "131",
pages = "1091--1103",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "3",

}

TY - JOUR

T1 - The Medicago species A2-type cyclin is auxin regulated and involved in meristem formation but dispensable for endoreduplication-associated developmental programs

AU - Roudier, François

AU - Fedorova, Elena

AU - Lebris, Manuel

AU - Lecomte, Phillippe

AU - Györgyey, J.

AU - Vaubert, Daniele

AU - Horváth, G.

AU - Abad, Pierre

AU - Kondorosi, Adam

AU - Kondorosi, É.

PY - 2003/3/1

Y1 - 2003/3/1

N2 - Phytohormones as well as temporal and spatial regulation of the cell cycle play a key role in plant development. Here, we investigated the function and regulation of an alfalfa (Medicago sativa) A2-type cyclin in three distinct root developmental programs: in primary and secondary root development, nodule development, and nematode-elicited gall formation. Using transgenic plants carrying the Medsa;cycA2;2 promoter-β-glucuronidase gene fusion, in combination with other techniques, cycA2;2 expression was localized in meristems and proliferating cells in the lateral root and nodule primordia. Rapid induction of cycA2;2 by Nod factors demonstrated that this gene is implicated in cell cycle activation of differentiated cells developing to nodule primordia. Surprisingly, cycA2;2 was repressed in the endoreduplicating, division-arrested cells both during nodule development and formation of giant cells in nematode-induced galls, indicating that CycA2;2 was dispensable for S-phase in endoreduplication cycles. Overexpression of cycA2;2 in transgenic plants corresponded to wild type protein levels and had no apparent phenotype. In contrast, antisense expression of cycA2;2 halted regeneration of somatic embryos, suggesting a role for CycA2;2 in the formation or activity of apical meristems. Expression of cycA2;2 was up-regulated by auxins, as expected from the presence of auxin response elements in the promoter. Moreover, auxin also affected the spatial expression pattern of this cyclin by shifting the cycA2;2 expression from the phloem to the xylem poles.

AB - Phytohormones as well as temporal and spatial regulation of the cell cycle play a key role in plant development. Here, we investigated the function and regulation of an alfalfa (Medicago sativa) A2-type cyclin in three distinct root developmental programs: in primary and secondary root development, nodule development, and nematode-elicited gall formation. Using transgenic plants carrying the Medsa;cycA2;2 promoter-β-glucuronidase gene fusion, in combination with other techniques, cycA2;2 expression was localized in meristems and proliferating cells in the lateral root and nodule primordia. Rapid induction of cycA2;2 by Nod factors demonstrated that this gene is implicated in cell cycle activation of differentiated cells developing to nodule primordia. Surprisingly, cycA2;2 was repressed in the endoreduplicating, division-arrested cells both during nodule development and formation of giant cells in nematode-induced galls, indicating that CycA2;2 was dispensable for S-phase in endoreduplication cycles. Overexpression of cycA2;2 in transgenic plants corresponded to wild type protein levels and had no apparent phenotype. In contrast, antisense expression of cycA2;2 halted regeneration of somatic embryos, suggesting a role for CycA2;2 in the formation or activity of apical meristems. Expression of cycA2;2 was up-regulated by auxins, as expected from the presence of auxin response elements in the promoter. Moreover, auxin also affected the spatial expression pattern of this cyclin by shifting the cycA2;2 expression from the phloem to the xylem poles.

UR - http://www.scopus.com/inward/record.url?scp=0037353416&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037353416&partnerID=8YFLogxK

U2 - 10.1104/pp.102.011122

DO - 10.1104/pp.102.011122

M3 - Article

VL - 131

SP - 1091

EP - 1103

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 3

ER -