The master regulator of IncA/C plasmids is recognized by the Salmonella Genomic island SGI1 as a signal for excision and conjugal transfer

János Kiss, Péter Pál Papp, Mónika Szabó, Tibor Farkas, Gábor Murányi, Erik Szakállas, Ferenc Olasz

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The genomic island SGI1 and its variants, the important vehicles of multi-resistance in Salmonella strains, are integrative elements mobilized exclusively by the conjugative IncA/C plasmids. Integration and excision of the island are carried out by the SGI1-encoded site-specific recombinase Int and the recombination directionality factor Xis. Chromosomal integration ensures the stable maintenance and vertical transmission of SGI1, while excision is the initial step of horizontal transfer, followed by conjugation and integration into the recipient. We report here that SGI1 not only exploits the conjugal apparatus of the IncA/C plasmids but also utilizes the regulatory mechanisms of the conjugation system for the exact timing and activation of excision to ensure efficient horizontal transfer. This study demonstrates that the FlhDC-family activator AcaCD, which regulates the conjugation machinery of the IncA/C plasmids, serves as a signal of helper entry through binding to SGI1 xis promoter and activating SGI1 excision. Promoters of int and xis genes have been identified and the binding site of the activator has been located by footprinting and deletion analyses. We prove that expression of xis is activator-dependent while int is constitutively expressed, and this regulatory mechanism is presumably responsible for the efficient transfer and stable maintenance of SGI1.

Original languageEnglish
Pages (from-to)8735-8745
Number of pages11
JournalNucleic Acids Research
Volume43
Issue number18
DOIs
Publication statusPublished - 2015

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Genomic Islands
Salmonella
Plasmids
Maintenance
Islands
Genetic Recombination
Binding Sites
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

The master regulator of IncA/C plasmids is recognized by the Salmonella Genomic island SGI1 as a signal for excision and conjugal transfer. / Kiss, János; Papp, Péter Pál; Szabó, Mónika; Farkas, Tibor; Murányi, Gábor; Szakállas, Erik; Olasz, Ferenc.

In: Nucleic Acids Research, Vol. 43, No. 18, 2015, p. 8735-8745.

Research output: Contribution to journalArticle

Kiss, János ; Papp, Péter Pál ; Szabó, Mónika ; Farkas, Tibor ; Murányi, Gábor ; Szakállas, Erik ; Olasz, Ferenc. / The master regulator of IncA/C plasmids is recognized by the Salmonella Genomic island SGI1 as a signal for excision and conjugal transfer. In: Nucleic Acids Research. 2015 ; Vol. 43, No. 18. pp. 8735-8745.
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