The localization of calcium by X-ray microanalysis in myopathic muscle fibers

Ernö Dux, I. Tóth, L. Dux, Ferenc Joó

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

An electron histochemical study was undertaken to localize calcium with ammonium oxalate precipitation technique in soleus muscle of rat in normal cases and in myopathy induced experimentally by a prolonged treatment of 2,4-dichlorophenoxyacetate (2,4-D). The calcium content of precipitates was detected by energy-dispersive X-ray microanalysis. In normal cases, the electron dense precipitates containing calcium were mainly found in the vesicles of sarcoplasmic reticulum, whereas in 2,4-D induced myopathy the deposits were shifted near the Z line into the myofibrils. Calcium, because the uptake into sarcoplasmic vesicles was inhibited by 2,4-D, could attach to other binding sites, such as to the troponin-C.A long-lasting binding of calcium might lead to a prolonged activation of the actin-myosin system.

Original languageEnglish
Pages (from-to)239-244
Number of pages6
JournalHistochemistry
Volume56
Issue number3-4
DOIs
Publication statusPublished - Sep 1978

Fingerprint

Electron Probe Microanalysis
2,4-Dichlorophenoxyacetic Acid
Calcium
Muscles
Muscular Diseases
Electrons
Oxalic Acid
Troponin
Myofibrils
Sarcoplasmic Reticulum
Myosins
Actins
Skeletal Muscle
Binding Sites

ASJC Scopus subject areas

  • Anatomy

Cite this

The localization of calcium by X-ray microanalysis in myopathic muscle fibers. / Dux, Ernö; Tóth, I.; Dux, L.; Joó, Ferenc.

In: Histochemistry, Vol. 56, No. 3-4, 09.1978, p. 239-244.

Research output: Contribution to journalArticle

@article{c49b75f13b6f4975b5498682fd80e7ad,
title = "The localization of calcium by X-ray microanalysis in myopathic muscle fibers",
abstract = "An electron histochemical study was undertaken to localize calcium with ammonium oxalate precipitation technique in soleus muscle of rat in normal cases and in myopathy induced experimentally by a prolonged treatment of 2,4-dichlorophenoxyacetate (2,4-D). The calcium content of precipitates was detected by energy-dispersive X-ray microanalysis. In normal cases, the electron dense precipitates containing calcium were mainly found in the vesicles of sarcoplasmic reticulum, whereas in 2,4-D induced myopathy the deposits were shifted near the Z line into the myofibrils. Calcium, because the uptake into sarcoplasmic vesicles was inhibited by 2,4-D, could attach to other binding sites, such as to the troponin-C.A long-lasting binding of calcium might lead to a prolonged activation of the actin-myosin system.",
author = "Ern{\"o} Dux and I. T{\'o}th and L. Dux and Ferenc Jo{\'o}",
year = "1978",
month = "9",
doi = "10.1007/BF00495985",
language = "English",
volume = "56",
pages = "239--244",
journal = "Histochemie. Histochemistry. Histochimie",
issn = "0948-6143",
publisher = "Springer Verlag",
number = "3-4",

}

TY - JOUR

T1 - The localization of calcium by X-ray microanalysis in myopathic muscle fibers

AU - Dux, Ernö

AU - Tóth, I.

AU - Dux, L.

AU - Joó, Ferenc

PY - 1978/9

Y1 - 1978/9

N2 - An electron histochemical study was undertaken to localize calcium with ammonium oxalate precipitation technique in soleus muscle of rat in normal cases and in myopathy induced experimentally by a prolonged treatment of 2,4-dichlorophenoxyacetate (2,4-D). The calcium content of precipitates was detected by energy-dispersive X-ray microanalysis. In normal cases, the electron dense precipitates containing calcium were mainly found in the vesicles of sarcoplasmic reticulum, whereas in 2,4-D induced myopathy the deposits were shifted near the Z line into the myofibrils. Calcium, because the uptake into sarcoplasmic vesicles was inhibited by 2,4-D, could attach to other binding sites, such as to the troponin-C.A long-lasting binding of calcium might lead to a prolonged activation of the actin-myosin system.

AB - An electron histochemical study was undertaken to localize calcium with ammonium oxalate precipitation technique in soleus muscle of rat in normal cases and in myopathy induced experimentally by a prolonged treatment of 2,4-dichlorophenoxyacetate (2,4-D). The calcium content of precipitates was detected by energy-dispersive X-ray microanalysis. In normal cases, the electron dense precipitates containing calcium were mainly found in the vesicles of sarcoplasmic reticulum, whereas in 2,4-D induced myopathy the deposits were shifted near the Z line into the myofibrils. Calcium, because the uptake into sarcoplasmic vesicles was inhibited by 2,4-D, could attach to other binding sites, such as to the troponin-C.A long-lasting binding of calcium might lead to a prolonged activation of the actin-myosin system.

UR - http://www.scopus.com/inward/record.url?scp=0018104810&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018104810&partnerID=8YFLogxK

U2 - 10.1007/BF00495985

DO - 10.1007/BF00495985

M3 - Article

C2 - 689920

AN - SCOPUS:0018104810

VL - 56

SP - 239

EP - 244

JO - Histochemie. Histochemistry. Histochimie

JF - Histochemie. Histochemistry. Histochimie

SN - 0948-6143

IS - 3-4

ER -