The Local Dynamics of the Active Site Region of Glycogen Phosphorylase b

B. Somogyi, A. Szarka, Z. Lakos

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The parameters characterizing the quenching of fluorescence emitted by the coenzyme (pyridoxal-5′-phosphate) of phosphorylase b (EC 2.4.1.1) by anions are good indicators of conformational/dynamic changes at the active center. Reinvestigation of this quenching process resulted in a non-linear Stern-Volmer plot. This non-linearity is described by a simple kinetic model which assumes two parallel processes, one represented by bound and the second by free quencher molecules. Analysis of separate parts of the nonlinear Stern-Volmer plot results in the values of rate constants for the bound and free quencher molecules as well as the value of dissociation constant of the anions.

Original languageEnglish
Pages (from-to)936-943
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume209
Issue number3
DOIs
Publication statusPublished - Apr 26 1995

Fingerprint

Phosphorylase b
Glycogen Phosphorylase
Anions
Quenching
Catalytic Domain
Molecules
Pyridoxal Phosphate
Coenzymes
Rate constants
Fluorescence
Kinetics

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology
  • Biophysics
  • Biochemistry

Cite this

The Local Dynamics of the Active Site Region of Glycogen Phosphorylase b. / Somogyi, B.; Szarka, A.; Lakos, Z.

In: Biochemical and Biophysical Research Communications, Vol. 209, No. 3, 26.04.1995, p. 936-943.

Research output: Contribution to journalArticle

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