The lipoxygenase product 13-hydroxyoctadecadienoic acid (13-HODE) is a selective inhibitor of classical PKC isoenzymes

J. Pongrácz, Janet M. Lord

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

13-Hydroxyoctadecadienoic acid (13-HODE), hydroxylinoleic acid, is a major lipoxygenase metabolite which is produced by myeloid inflammatory cells and modifies inflammatory cell activity. The biological effects of 13-HODE in non-haemopoietic cells (HUVEC) have been attributed to the incorporation of 13-HODE into 13-HODE containing diacylglycerol and the selective inhibition of protein kinase C. Our studies, using whole promyeloid cells (HL60) and recombinant PKC isoenzymes in an in vitro assay, showed that 13-HODE inhibited PKC-α, β1, and PKC-β11, but did not affect the activity of PKC-δ. These data suggest that the actions of hydroxylinoleic acid on myeloid cells include the selective inhibition of classical PKC isoenzymes.

Original languageEnglish
Pages (from-to)269-272
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume256
Issue number2
DOIs
Publication statusPublished - Mar 16 1999

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Lipoxygenase
Isoenzymes
Myeloid Cells
Acids
HL-60 Cells
Diglycerides
Human Umbilical Vein Endothelial Cells
Metabolites
Protein Kinase C
Assays
13-hydroxy-9,11-octadecadienoic acid

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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abstract = "13-Hydroxyoctadecadienoic acid (13-HODE), hydroxylinoleic acid, is a major lipoxygenase metabolite which is produced by myeloid inflammatory cells and modifies inflammatory cell activity. The biological effects of 13-HODE in non-haemopoietic cells (HUVEC) have been attributed to the incorporation of 13-HODE into 13-HODE containing diacylglycerol and the selective inhibition of protein kinase C. Our studies, using whole promyeloid cells (HL60) and recombinant PKC isoenzymes in an in vitro assay, showed that 13-HODE inhibited PKC-α, β1, and PKC-β11, but did not affect the activity of PKC-δ. These data suggest that the actions of hydroxylinoleic acid on myeloid cells include the selective inhibition of classical PKC isoenzymes.",
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