The ligand-binding domain of the cell surface receptor for urokinase-type plasminogen activator

Niels Behrendt, Michael Ploug, L. Patthy, Gunnar Houen, Francesco Blasi, Keld Danø

Research output: Contribution to journalArticle

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Abstract

The purified urokinase plasminogen activator receptor (u-PAR) was cleaved into two fragments by mild chymotrypsin treatment. The smaller fragment (apparent Mr 16,000) possessed the ligand-binding capability, as shown by chemical cross-linking analysis. This fragment constituted the NH2-terminal part of the intact receptor, probably including the whole sequence 1-87, and contained N-linked carbohydrate. After detergent phase separation in the Triton X-114 system, the fragment was present in the water phase where its binding activity could be demonstrated in the absence of the rest of the protein. An analysis of internal homology in the amino acid sequence of u-PAR revealed the presence of three repeats of approximately 90 residues each. The ligand-binding fragment corresponds to the first repeat, supporting that this unit is a structurally autonomous domain. Domains homologous with the internal repeats of u-PAR constitute the extracellular part of Ly-6 antigens and of the squid glycoprotein Sgp-2. Like u-PAR, these proteins are attached to the membrane by a glycosyl-phosphatidylinositol anchor. The hydrophilic, ligand-binding u-PAR domain identified in the present study has potential applications in interfering with cell-surface plasmin-mediated proteolysis.

Original languageEnglish
Pages (from-to)7842-7847
Number of pages6
JournalJournal of Biological Chemistry
Volume266
Issue number12
Publication statusPublished - Apr 25 1991

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Urokinase Plasminogen Activator Receptors
Urokinase-Type Plasminogen Activator
Cell Surface Receptors
Ligands
Ly Antigens
Proteolysis
Amino Acid Sequence Homology
Decapodiformes
Glycosylphosphatidylinositols
Fibrinolysin
Chymotrypsin
Anchors
Phase separation
Detergents
Glycoproteins
Proteins
Carbohydrates
Membranes
Antigens
Amino Acids

ASJC Scopus subject areas

  • Biochemistry

Cite this

The ligand-binding domain of the cell surface receptor for urokinase-type plasminogen activator. / Behrendt, Niels; Ploug, Michael; Patthy, L.; Houen, Gunnar; Blasi, Francesco; Danø, Keld.

In: Journal of Biological Chemistry, Vol. 266, No. 12, 25.04.1991, p. 7842-7847.

Research output: Contribution to journalArticle

Behrendt, N, Ploug, M, Patthy, L, Houen, G, Blasi, F & Danø, K 1991, 'The ligand-binding domain of the cell surface receptor for urokinase-type plasminogen activator', Journal of Biological Chemistry, vol. 266, no. 12, pp. 7842-7847.
Behrendt, Niels ; Ploug, Michael ; Patthy, L. ; Houen, Gunnar ; Blasi, Francesco ; Danø, Keld. / The ligand-binding domain of the cell surface receptor for urokinase-type plasminogen activator. In: Journal of Biological Chemistry. 1991 ; Vol. 266, No. 12. pp. 7842-7847.
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