The latent membrane protein-1 in Epstein-Barr virus-transformed lymphoblastoid cells is found with ubiquitin-protein conjugates and heat-shock protein 70 in lysosomes oriented around the microtubule organizing centre

L. László, J. Tuckwell, T. Self, J. Lowe, M. Landon, S. Smith, J. N. Hawthorne, R. J. Mayer

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Immunofluorescence studies on Epstein-Barr virus (EBV)-transformed lymphoblastoid cells have previously shown that the latent membrane transforming protein (LMP-1) is found in patch-like inclusions which also immunostain for vimentin. We now show that EBV transformation causes a major reorganization of intermediate filaments, micro-tubules, mitochondria, and lysosomal elements, which generally become oriented around the microtubule organizing centre. Immunogold electron microscopy shows that LMP-1 is primarily concentrated in secondary lysosomes together with ubiquitin-protein conjugates and heat-shock protein 70. Intermediate filament inclusion formation with the above characteristics may be a general response triggered by other membrane glycoproteins; as seen, for example, in major human neurodegenerative diseases such as diffuse Lewy body disease.

Original languageEnglish
Pages (from-to)203-214
Number of pages12
JournalJournal of Pathology
Volume164
Issue number3
Publication statusPublished - 1991

Fingerprint

Microtubule-Organizing Center
HSP70 Heat-Shock Proteins
Intermediate Filaments
Ubiquitin
Lysosomes
Human Herpesvirus 4
Lewy Body Disease
Membrane Glycoproteins
Vimentin
Neurodegenerative Diseases
Fluorescent Antibody Technique
Electron Microscopy
Mitochondria
Membrane Proteins
Proteins
Epstein-Barr virus EBV-associated membrane antigen

Keywords

  • EBV latent membrane protein
  • Heat-shock proteins
  • Intermediate filaments
  • Lysosomes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

The latent membrane protein-1 in Epstein-Barr virus-transformed lymphoblastoid cells is found with ubiquitin-protein conjugates and heat-shock protein 70 in lysosomes oriented around the microtubule organizing centre. / László, L.; Tuckwell, J.; Self, T.; Lowe, J.; Landon, M.; Smith, S.; Hawthorne, J. N.; Mayer, R. J.

In: Journal of Pathology, Vol. 164, No. 3, 1991, p. 203-214.

Research output: Contribution to journalArticle

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AU - Tuckwell, J.

AU - Self, T.

AU - Lowe, J.

AU - Landon, M.

AU - Smith, S.

AU - Hawthorne, J. N.

AU - Mayer, R. J.

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