The latent membrane protein-1 in Epstein-Barr virus-transformed lymphoblastoid cells is found with ubiquitin-protein conjugates and heat-shock protein 70 in lysosomes oriented around the microtubule organizing centre

L. László, J. Tuckwell, T. Self, J. Lowe, M. Landon, S. Smith, J. N. Hawthorne, R. J. Mayer

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Abstract

Immunofluorescence studies on Epstein-Barr virus (EBV)-transformed lymphoblastoid cells have previously shown that the latent membrane transforming protein (LMP-1) is found in patch-like inclusions which also immunostain for vimentin. We now show that EBV transformation causes a major reorganization of intermediate filaments, micro-tubules, mitochondria, and lysosomal elements, which generally become oriented around the microtubule organizing centre. Immunogold electron microscopy shows that LMP-1 is primarily concentrated in secondary lysosomes together with ubiquitin-protein conjugates and heat-shock protein 70. Intermediate filament inclusion formation with the above characteristics may be a general response triggered by other membrane glycoproteins; as seen, for example, in major human neurodegenerative diseases such as diffuse Lewy body disease.

Original languageEnglish
Pages (from-to)203-214
Number of pages12
JournalJournal of Pathology
Volume164
Issue number3
Publication statusPublished - 1991

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Keywords

  • EBV latent membrane protein
  • Heat-shock proteins
  • Intermediate filaments
  • Lysosomes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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