The Ketel gene encodes a Drosophila homologue of importin-β

M. Lippai, L. Tirián, I. Boros, J. Mihaly, M. Erdélyi, I. Belecz, E. Mathe, J. Posfai, A. Nagy, A. Udvardy, E. Paraskeva, D. Gorlich, J. Szabad

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Abstract

The Drosophila melanogaster Ketel gene was identified via the Ketel(D) dominant female sterile mutations and their ketel(r) revertant alleles that are recessive zygotic lethals. The maternally acting Ketel(D) mutations inhibit cleavage nuclei formation. We cloned the Ketel gene on the basis of a common breakpoint in 38E1.2-3 in four ketel(r) alleles. The Ketel+ transgenes rescue ketel(r)-associated zygotic lethality and slightly reduce Ketel(D)-associated dominant female sterility. Ketel is a single copy gene. It is transcribed to a single 3.6-kb mRNA, predicted to encode the 97-kD Ketel protein. The 884-amino-acid sequence of Ketel is 60% identical and 78% similar to that of human importin-β, the nuclear import receptor for proteins with a classical NLS. Indeed, Ketel supports import of appropriately designed substrates into nuclei of digitonin-permeabilized HeLa cells As shown by a polyclonal anti-Ketel antibody, nurse cells synthesize and transfer Ketel protein into the oocyte cytoplasm from stage 11 of oogenesis. In cleavage embryos the Ketel protein is cytoplasmic. The Ketel gene appears to be ubiquitously expressed in embryonic cells. Western blot analysis revealed that the Ketel gene is not expressed in several larval cell types of late third instar larvae.

Original languageEnglish
Pages (from-to)1889-1900
Number of pages12
JournalGenetics
Volume156
Issue number4
Publication statusPublished - 2000

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Karyopherins
Drosophila
Genes
Proteins
Alleles
Female Infertility
Digitonin
Oogenesis
Mutation
Cell Nucleus Active Transport
Cytoplasmic and Nuclear Receptors
Drosophila melanogaster
Transgenes
HeLa Cells
Oocytes
Larva
Anti-Idiotypic Antibodies
Amino Acid Sequence
Cytoplasm
Embryonic Structures

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

The Ketel gene encodes a Drosophila homologue of importin-β. / Lippai, M.; Tirián, L.; Boros, I.; Mihaly, J.; Erdélyi, M.; Belecz, I.; Mathe, E.; Posfai, J.; Nagy, A.; Udvardy, A.; Paraskeva, E.; Gorlich, D.; Szabad, J.

In: Genetics, Vol. 156, No. 4, 2000, p. 1889-1900.

Research output: Contribution to journalArticle

Lippai, M, Tirián, L, Boros, I, Mihaly, J, Erdélyi, M, Belecz, I, Mathe, E, Posfai, J, Nagy, A, Udvardy, A, Paraskeva, E, Gorlich, D & Szabad, J 2000, 'The Ketel gene encodes a Drosophila homologue of importin-β', Genetics, vol. 156, no. 4, pp. 1889-1900.
Lippai, M. ; Tirián, L. ; Boros, I. ; Mihaly, J. ; Erdélyi, M. ; Belecz, I. ; Mathe, E. ; Posfai, J. ; Nagy, A. ; Udvardy, A. ; Paraskeva, E. ; Gorlich, D. ; Szabad, J. / The Ketel gene encodes a Drosophila homologue of importin-β. In: Genetics. 2000 ; Vol. 156, No. 4. pp. 1889-1900.
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AU - Tirián, L.

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AU - Erdélyi, M.

AU - Belecz, I.

AU - Mathe, E.

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AB - The Drosophila melanogaster Ketel gene was identified via the Ketel(D) dominant female sterile mutations and their ketel(r) revertant alleles that are recessive zygotic lethals. The maternally acting Ketel(D) mutations inhibit cleavage nuclei formation. We cloned the Ketel gene on the basis of a common breakpoint in 38E1.2-3 in four ketel(r) alleles. The Ketel+ transgenes rescue ketel(r)-associated zygotic lethality and slightly reduce Ketel(D)-associated dominant female sterility. Ketel is a single copy gene. It is transcribed to a single 3.6-kb mRNA, predicted to encode the 97-kD Ketel protein. The 884-amino-acid sequence of Ketel is 60% identical and 78% similar to that of human importin-β, the nuclear import receptor for proteins with a classical NLS. Indeed, Ketel supports import of appropriately designed substrates into nuclei of digitonin-permeabilized HeLa cells As shown by a polyclonal anti-Ketel antibody, nurse cells synthesize and transfer Ketel protein into the oocyte cytoplasm from stage 11 of oogenesis. In cleavage embryos the Ketel protein is cytoplasmic. The Ketel gene appears to be ubiquitously expressed in embryonic cells. Western blot analysis revealed that the Ketel gene is not expressed in several larval cell types of late third instar larvae.

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