The isolation of viable egg cells of wheat (Triticum aestivum L.)

Miklós Kovács, Beáta Barnabás, Erhard Kranz

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The isolation of viable egg cells of wheat has been achieved without enzymatic maceration of the ovules. 2,4-D applied to the stigmas resulted 3 to 7 days later in soft ovule tissues which disintegrated upon mechanical manipulation. The isolated egg cells were viable even 2 h after isolation. Their morphology corresponded to that of the in situ egg cells. The mean isolation frequency was 20% (two egg cells per ten ovules).

Original languageEnglish
Pages (from-to)311-312
Number of pages2
JournalSexual Plant Reproduction
Volume7
Issue number5
DOIs
Publication statusPublished - Sep 1 1994

Keywords

  • Egg cell apparatus in situ Egg cell isolation
  • Wheat

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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