The isolation of Nε{lunate}-formyl-l-lysine from the reaction between formaldehyde and l-lysine and its identification by OPLC and NMR spectroscopy

E. Tyihák, Lajos Trézl, P. Kolonits

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

From the reaction between l-lysine and formaldehyde, Nε{lunate}-formyl-l-lysine was isolated by means of ion-exchange column chromatography. The identification of Nε{lunate}-formyl-l-lysine was carried out by ion-exchange overpressured-layer chromatography (OPLC) and 1H NMR and 13C NMR spectroscopies. The m.p. and mixed m.p. values, the retention characteristics and the chemical shifts of the isolated product were identical with those of an authentic sample of Nε{lunate}-formyl-l-lysine.

Original languageEnglish
Pages (from-to)343-349
Number of pages7
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume3
Issue number4
DOIs
Publication statusPublished - 1985

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Chromatography
Formaldehyde
Nuclear magnetic resonance spectroscopy
Lysine
Magnetic Resonance Spectroscopy
Ion exchange
Column chromatography
Ion Exchange
Ion Exchange Chromatography
Chemical shift
Nuclear magnetic resonance

Keywords

  • C NMR spectroscopy.
  • H NMR spectroscopy
  • N-formyl-l-lysine
  • overpressured-layer chromatography (OPLC)
  • Spontaneous formylation of l-lysine with formaldehyde

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science

Cite this

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title = "The isolation of Nε{lunate}-formyl-l-lysine from the reaction between formaldehyde and l-lysine and its identification by OPLC and NMR spectroscopy",
abstract = "From the reaction between l-lysine and formaldehyde, Nε{lunate}-formyl-l-lysine was isolated by means of ion-exchange column chromatography. The identification of Nε{lunate}-formyl-l-lysine was carried out by ion-exchange overpressured-layer chromatography (OPLC) and 1H NMR and 13C NMR spectroscopies. The m.p. and mixed m.p. values, the retention characteristics and the chemical shifts of the isolated product were identical with those of an authentic sample of Nε{lunate}-formyl-l-lysine.",
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T1 - The isolation of Nε{lunate}-formyl-l-lysine from the reaction between formaldehyde and l-lysine and its identification by OPLC and NMR spectroscopy

AU - Tyihák, E.

AU - Trézl, Lajos

AU - Kolonits, P.

PY - 1985

Y1 - 1985

N2 - From the reaction between l-lysine and formaldehyde, Nε{lunate}-formyl-l-lysine was isolated by means of ion-exchange column chromatography. The identification of Nε{lunate}-formyl-l-lysine was carried out by ion-exchange overpressured-layer chromatography (OPLC) and 1H NMR and 13C NMR spectroscopies. The m.p. and mixed m.p. values, the retention characteristics and the chemical shifts of the isolated product were identical with those of an authentic sample of Nε{lunate}-formyl-l-lysine.

AB - From the reaction between l-lysine and formaldehyde, Nε{lunate}-formyl-l-lysine was isolated by means of ion-exchange column chromatography. The identification of Nε{lunate}-formyl-l-lysine was carried out by ion-exchange overpressured-layer chromatography (OPLC) and 1H NMR and 13C NMR spectroscopies. The m.p. and mixed m.p. values, the retention characteristics and the chemical shifts of the isolated product were identical with those of an authentic sample of Nε{lunate}-formyl-l-lysine.

KW - C NMR spectroscopy.

KW - H NMR spectroscopy

KW - N-formyl-l-lysine

KW - overpressured-layer chromatography (OPLC)

KW - Spontaneous formylation of l-lysine with formaldehyde

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