The isolation of Nϵ-formyl-l-lysine from the reaction between formaldehyde and l-lysine and its identification by OPLC and NMR spectroscopy

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Abstract

From the reaction between l-lysine and formaldehyde, Nε{lunate}-formyl-l-lysine was isolated by means of ion-exchange column chromatography. The identification of Nε{lunate}-formyl-l-lysine was carried out by ion-exchange overpressured-layer chromatography (OPLC) and 1H NMR and 13C NMR spectroscopies. The m.p. and mixed m.p. values, the retention characteristics and the chemical shifts of the isolated product were identical with those of an authentic sample of Nε{lunate}-formyl-l-lysine.

Original languageEnglish
Pages (from-to)343-349
Number of pages7
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume3
Issue number4
DOIs
Publication statusPublished - 1985

Keywords

  • C NMR spectroscopy.
  • H NMR spectroscopy
  • N-formyl-l-lysine
  • Spontaneous formylation of l-lysine with formaldehyde
  • overpressured-layer chromatography (OPLC)

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

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