The IP amplitude of the fluorescence rise OJIP is sensitive to changes in the photosystem I content of leaves

A study on plants exposed to magnesium and sulfate deficiencies, drought stress and salt stress

Margarita Georgina Ceppi, Abdallah Oukarroum, Nuran Çiçek, Reto J. Strasser, G. Schansker

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

The hypothesis that changes in the IP amplitude of the fluorescence transient OJIP reflect changes in leaf photosystem I (PSI) content was tested using mineral-deficient sugar beet plants. Young sugar beet plants (Beta vulgaris) were grown hydroponically on nutrient solutions containing either 1 mM or no Mg 2+ and 2.1 μM to 1.88 mM SO 4 2- for 4 weeks. During this period two leaf pairs were followed: the already developed second leaf pair and the third leaf pair that was budding at the start of the treatment. The IP amplitude [ΔF IP (fluorescence amplitude of the I-to-P-rise) and its relative contribution to the fluorescence rise: ΔV IP (amplitude of the relative variable fluorescence of the I-to-P-rise = relative contribution of the I-to-P-rise to the OJIP-rise)] and the amplitude of the transmission change at 820 nm (difference between all plastocyanin and the primary electron donor of photosystems I oxidized and reduced, respectively) relative to the total transmission signal (ΔI max/I tot) were determined as a function of the treatment time. Correlating the transmission and the two fluorescence parameters yielded approximately linear relationships in both cases. For the least severely affected leaves the parameter ΔV IP correlated considerably better with ΔI max/I tot than ΔF IP indicating that it is the ratio PSII:PSI that counts. To show that the relationship also holds for other plants and treatments, data from salt- and drought-stressed plants of barley, chickpea and pea are shown. The relationship between ΔV IP and PSI content was confirmed by western blot analysis using an antibody against psaD. The good correlations between ΔI max/I tot and ΔF IP and ΔV IP, respectively, suggest that changes in the IP amplitude can be used as semi-quantitative indicators for (relative) changes in the PSI content of the leaf.

Original languageEnglish
Pages (from-to)277-288
Number of pages12
JournalPhysiologia Plantarum
Volume144
Issue number3
DOIs
Publication statusPublished - Mar 2012

Fingerprint

Magnesium Deficiency
Photosystem I Protein Complex
Magnesium Sulfate
Droughts
photosystem I
salt stress
magnesium
sulfates
water stress
Salts
Fluorescence
Beta vulgaris
fluorescence
leaves
sugar beet
Plastocyanin
Cicer
plastocyanin
Peas
Hordeum

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics
  • Physiology

Cite this

The IP amplitude of the fluorescence rise OJIP is sensitive to changes in the photosystem I content of leaves : A study on plants exposed to magnesium and sulfate deficiencies, drought stress and salt stress. / Ceppi, Margarita Georgina; Oukarroum, Abdallah; Çiçek, Nuran; Strasser, Reto J.; Schansker, G.

In: Physiologia Plantarum, Vol. 144, No. 3, 03.2012, p. 277-288.

Research output: Contribution to journalArticle

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