The involvement of ATP sulfurylase in Se(VI) and Cr(VI) reduction processes in the fission yeast Schizosaccharomyces pombe

P. Raspor, Š Fujs, L. Banszky, A. Maráz, M. Batič

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The response of Schizosaccharomyces pombe towards the oxyanions selenate [Se(VI)] and dichromate [Cr(VI)] was investigated in order to establish the involvement of the yeast ATP sulfurylase in their reduction. An ATP sulfurylase-defective/selenate-resistant mutant of S. pombe (B-579 Se R-2) and an ATP sulfurylase-active/selenate-sensitive strain of S. pombe (B-579 SeS) were included in this study. The inhibitory effect of Se(VI) and Cr(VI) oxyanions on growth and bioaccumulation was measured. The sensitive strain showed natural sensitivity to selenate while the resistant mutant tolerated a 100-fold higher concentration of selenate. These results indicate that selenate toxicity to microorganisms is connected with the reduction of selenate to selenite. Both strains showed similar sensitivity to Cr(VI) and in this study there was no evidence that ATP sulfurylase participates in the reduction process of Cr(VI).

Original languageEnglish
Pages (from-to)89-95
Number of pages7
JournalApplied Microbiology and Biotechnology
Volume63
Issue number1
DOIs
Publication statusPublished - Nov 2003

Fingerprint

Sulfate Adenylyltransferase
Selenic Acid
Schizosaccharomyces
Adenosinetriphosphate
Yeast
Bioaccumulation
Microorganisms
Toxicity
Selenious Acid
chromium hexavalent ion
Yeasts

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Bioengineering

Cite this

The involvement of ATP sulfurylase in Se(VI) and Cr(VI) reduction processes in the fission yeast Schizosaccharomyces pombe. / Raspor, P.; Fujs, Š; Banszky, L.; Maráz, A.; Batič, M.

In: Applied Microbiology and Biotechnology, Vol. 63, No. 1, 11.2003, p. 89-95.

Research output: Contribution to journalArticle

@article{0b9759e764cc4aa9bdf8bb29c87f4005,
title = "The involvement of ATP sulfurylase in Se(VI) and Cr(VI) reduction processes in the fission yeast Schizosaccharomyces pombe",
abstract = "The response of Schizosaccharomyces pombe towards the oxyanions selenate [Se(VI)] and dichromate [Cr(VI)] was investigated in order to establish the involvement of the yeast ATP sulfurylase in their reduction. An ATP sulfurylase-defective/selenate-resistant mutant of S. pombe (B-579 Se R-2) and an ATP sulfurylase-active/selenate-sensitive strain of S. pombe (B-579 SeS) were included in this study. The inhibitory effect of Se(VI) and Cr(VI) oxyanions on growth and bioaccumulation was measured. The sensitive strain showed natural sensitivity to selenate while the resistant mutant tolerated a 100-fold higher concentration of selenate. These results indicate that selenate toxicity to microorganisms is connected with the reduction of selenate to selenite. Both strains showed similar sensitivity to Cr(VI) and in this study there was no evidence that ATP sulfurylase participates in the reduction process of Cr(VI).",
author = "P. Raspor and Š Fujs and L. Banszky and A. Mar{\'a}z and M. Batič",
year = "2003",
month = "11",
doi = "10.1007/s00253-003-1336-1",
language = "English",
volume = "63",
pages = "89--95",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - The involvement of ATP sulfurylase in Se(VI) and Cr(VI) reduction processes in the fission yeast Schizosaccharomyces pombe

AU - Raspor, P.

AU - Fujs, Š

AU - Banszky, L.

AU - Maráz, A.

AU - Batič, M.

PY - 2003/11

Y1 - 2003/11

N2 - The response of Schizosaccharomyces pombe towards the oxyanions selenate [Se(VI)] and dichromate [Cr(VI)] was investigated in order to establish the involvement of the yeast ATP sulfurylase in their reduction. An ATP sulfurylase-defective/selenate-resistant mutant of S. pombe (B-579 Se R-2) and an ATP sulfurylase-active/selenate-sensitive strain of S. pombe (B-579 SeS) were included in this study. The inhibitory effect of Se(VI) and Cr(VI) oxyanions on growth and bioaccumulation was measured. The sensitive strain showed natural sensitivity to selenate while the resistant mutant tolerated a 100-fold higher concentration of selenate. These results indicate that selenate toxicity to microorganisms is connected with the reduction of selenate to selenite. Both strains showed similar sensitivity to Cr(VI) and in this study there was no evidence that ATP sulfurylase participates in the reduction process of Cr(VI).

AB - The response of Schizosaccharomyces pombe towards the oxyanions selenate [Se(VI)] and dichromate [Cr(VI)] was investigated in order to establish the involvement of the yeast ATP sulfurylase in their reduction. An ATP sulfurylase-defective/selenate-resistant mutant of S. pombe (B-579 Se R-2) and an ATP sulfurylase-active/selenate-sensitive strain of S. pombe (B-579 SeS) were included in this study. The inhibitory effect of Se(VI) and Cr(VI) oxyanions on growth and bioaccumulation was measured. The sensitive strain showed natural sensitivity to selenate while the resistant mutant tolerated a 100-fold higher concentration of selenate. These results indicate that selenate toxicity to microorganisms is connected with the reduction of selenate to selenite. Both strains showed similar sensitivity to Cr(VI) and in this study there was no evidence that ATP sulfurylase participates in the reduction process of Cr(VI).

UR - http://www.scopus.com/inward/record.url?scp=1642562853&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1642562853&partnerID=8YFLogxK

U2 - 10.1007/s00253-003-1336-1

DO - 10.1007/s00253-003-1336-1

M3 - Article

VL - 63

SP - 89

EP - 95

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 1

ER -