The intracellular tyrosine residues of the ATP-gated P2X1 ion channel are essential for its function

E. Tóth-Zsámboki, Cecile Oury, Hiroyuki Watanabe, Bernd Nilius, Jos Vermylen, Marc F. Hoylaerts

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The four highly conserved intracellular tyrosine residues of the P2X1 ion channel were mutated into phenylalanine. Simultaneous electrophysiological and calcium measurements in transfected human embryonic kidney (HEK 293) cells indicated that Y362F and Y370F mutants were non-functional, despite their proper plasma membrane expression. The Y16F and Y363F mutants retained 2.2% and 26% of the wild-type P2X1 activity, respectively. However, no tyrosine phosphorylation was detected on Western blots of P2X1 immunoprecipitates derived either from HEK 293 cell lysates or from human platelets, expressing P2X1 endogenously. Thus, Y16, Y362, Y363 and Y370 are required for the appropriate three-dimensional structure and function of the intracellular P2X1 domains.

Original languageEnglish
Pages (from-to)15-19
Number of pages5
JournalFEBS Letters
Volume524
Issue number1-3
DOIs
Publication statusPublished - Jul 31 2002

Fingerprint

HEK293 Cells
Ion Channels
Tyrosine
Adenosine Triphosphate
Phosphorylation
Cell membranes
Platelets
Phenylalanine
Blood Platelets
Western Blotting
Cell Membrane
Calcium
Kidney

Keywords

  • ATP-gated non-selective cation channel
  • P2X receptor
  • Site-directed mutagenesis
  • Three-dimensional structure
  • Tyrosine residue

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The intracellular tyrosine residues of the ATP-gated P2X1 ion channel are essential for its function. / Tóth-Zsámboki, E.; Oury, Cecile; Watanabe, Hiroyuki; Nilius, Bernd; Vermylen, Jos; Hoylaerts, Marc F.

In: FEBS Letters, Vol. 524, No. 1-3, 31.07.2002, p. 15-19.

Research output: Contribution to journalArticle

Tóth-Zsámboki, E. ; Oury, Cecile ; Watanabe, Hiroyuki ; Nilius, Bernd ; Vermylen, Jos ; Hoylaerts, Marc F. / The intracellular tyrosine residues of the ATP-gated P2X1 ion channel are essential for its function. In: FEBS Letters. 2002 ; Vol. 524, No. 1-3. pp. 15-19.
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AU - Vermylen, Jos

AU - Hoylaerts, Marc F.

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