The interplay between tissue plasminogen activator domains and fibrin structures in the regulation of fibrinolysis: Kinetic and microscopic studies

Colin Longstaff, Craig Thelwell, Stella C. Williams, Marta M C G Silva, László Szabó, K. Kolev

Research output: Contribution to journalArticle

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Abstract

Regulation of tissue-type plasminogen activator (tPA) depends on fibrin binding and fibrin structure. tPA structure/function relationships were investigated in fibrin formed by high or low thrombin concentrations to produce a fine mesh and small pores, or thick fibers and coarse structure, respectively. Kinetics studies were performed to investigate plasminogen activation and fibrinolysis in the 2 types of fibrin, using wild-type tPA (F-G-K1-K2-P, F and K2 binding), K1K1-tPA (F-G-K1-K1-P, F binding), and delF-tPA (G-K1-K2-P, K2 binding). There was a trend of enzyme potency of tPA > K1K1-tPA > delF-tPA, highlighting the importance of the finger domain in regulating activity, but the differences were less apparent in fine fibrin. Fine fibrin was a better surface for plasminogen activation but more resistant to lysis. Scanning electron and confocal microscopy using orange fluorescent fibrin with green fluorescent protein-labeled tPA variants showed that tPA was strongly associated with agglomerates in coarse but not in fine fibrin. In later lytic stages, delF-tPA-green fluorescent protein diffused more rapidly through fibrin in contrast to full-length tPA, highlighting the importance of finger domainagglomerate interactions. Thus, the regulation of fibrinolysis depends on the starting nature of fibrin fibers and complex dynamic interaction between tPA and fibrin structures that vary over time.

Original languageEnglish
Pages (from-to)661-668
Number of pages8
JournalBlood
Volume117
Issue number2
DOIs
Publication statusPublished - Jan 13 2011

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Plasminogen Activators
Fibrinolysis
Tissue Plasminogen Activator
Fibrin
Kinetics
Plasminogen
Green Fluorescent Proteins
Fingers
Chemical activation
Fibers
Confocal microscopy
Confocal Microscopy
Thrombin
Electron Microscopy

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

The interplay between tissue plasminogen activator domains and fibrin structures in the regulation of fibrinolysis : Kinetic and microscopic studies. / Longstaff, Colin; Thelwell, Craig; Williams, Stella C.; Silva, Marta M C G; Szabó, László; Kolev, K.

In: Blood, Vol. 117, No. 2, 13.01.2011, p. 661-668.

Research output: Contribution to journalArticle

Longstaff, Colin ; Thelwell, Craig ; Williams, Stella C. ; Silva, Marta M C G ; Szabó, László ; Kolev, K. / The interplay between tissue plasminogen activator domains and fibrin structures in the regulation of fibrinolysis : Kinetic and microscopic studies. In: Blood. 2011 ; Vol. 117, No. 2. pp. 661-668.
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