The interleukin-8 receptor

A potential target for antipsoriatic therapy

L. Kemény, A. S. Kenderessy, E. Olasz, G. Michel, T. Ruzicka, A. Dobozy

Research output: Contribution to journalArticle

Abstract

Interleukin-8 (IL-8) is assumed to play a central role in the pathogenesis of inflammatory skin diseases, such as psoriasis. Since an increased expression of the IL-8 receptor (IL-8R) has been observed both in polymorphonuclear leukocytes (PMNL) and in involved psoriatic epidermis, we were interested whether the IL-8R could be the molecular target of antipsoriatic compounds. The IL-8R was detected on cultured normal human keratinocytes by means of flow cytometry using phycoery-thrine-labelled IL-8 (IL-8-PE). Pretreatment of keratinocytes for 24 h with cyclosporine, calcitriol, calcipotriol or dithranol resulted in a dose-dependent decrease of IL-8-PE binding to the cells, which was probably due to down-regulation of the binding sites. To establish whether the biologic function of the IL-8R was also affected, the effects of these substances on the IL-8-induced HLA-DR expression of keratinocytes was also studied. The IL-8-induced HLA-DR expression was nearly completely blocked by treatment of the cells with cyclosporine, calcitriol, calcipotriol or dithranol. On the other hand, IL-8 binding to PMNL was not affected by these antipsoriatic compounds, as measured in radioligand binding assays using 125I-IL-8. Inhibition of the keratinocyte IL-8R and function by antipsoriatic drugs may contribute to their therapeutic action.

Original languageEnglish
Pages (from-to)619-628
Number of pages10
JournalChronica Dermatologica
Volume4
Issue number4
Publication statusPublished - 1994

Fingerprint

Interleukin-8 Receptors
Interleukin-8
Keratinocytes
Anthralin
Calcitriol
HLA-DR Antigens
Therapeutics
Cyclosporine
Neutrophils
Radioligand Assay
Psoriasis
Skin Diseases
Epidermis
Flow Cytometry
Down-Regulation
Binding Sites

Keywords

  • calcitriol
  • cyclosporine
  • dithranol
  • interleukin-8 receptor
  • psoriasis

ASJC Scopus subject areas

  • Dermatology

Cite this

Kemény, L., Kenderessy, A. S., Olasz, E., Michel, G., Ruzicka, T., & Dobozy, A. (1994). The interleukin-8 receptor: A potential target for antipsoriatic therapy. Chronica Dermatologica, 4(4), 619-628.

The interleukin-8 receptor : A potential target for antipsoriatic therapy. / Kemény, L.; Kenderessy, A. S.; Olasz, E.; Michel, G.; Ruzicka, T.; Dobozy, A.

In: Chronica Dermatologica, Vol. 4, No. 4, 1994, p. 619-628.

Research output: Contribution to journalArticle

Kemény, L, Kenderessy, AS, Olasz, E, Michel, G, Ruzicka, T & Dobozy, A 1994, 'The interleukin-8 receptor: A potential target for antipsoriatic therapy', Chronica Dermatologica, vol. 4, no. 4, pp. 619-628.
Kemény, L. ; Kenderessy, A. S. ; Olasz, E. ; Michel, G. ; Ruzicka, T. ; Dobozy, A. / The interleukin-8 receptor : A potential target for antipsoriatic therapy. In: Chronica Dermatologica. 1994 ; Vol. 4, No. 4. pp. 619-628.
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