The Insulin Receptor Is Colocalized with the TRPV1 Nociceptive Ion Channel and Neuropeptides in Pancreatic Spinal and Vagal Primary Sensory Neurons

Bence András Lázár, G. Jancsó, Orsolya Oszlács, István Nagy, P. Sántha

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objectives Recent observations demonstrated the expression of the insulin receptor (InsR) and its functional interaction with the transient receptor potential vanilloid type 1 receptor (TRPV1) in sensory ganglion neurons. Because sensory nerves are implicated in pancreatic inflammatory processes, we studied the colocalization of the InsR with TRPV1 and proinflammatory neuropeptides in spinal and vagal pancreatic afferent neurons. Methods Immunohistochemistry and quantitative morphometry were used to analyze the expression of TRPV1, InsR, substance P (SP), and calcitonin gene-related peptide (CGRP) in retrogradely labeled pancreatic dorsal root ganglion (DRG) and nodose ganglion (NG) neurons. Results The proportions of retrogradely labeled pancreatic TRPV1-, InsR-, SP-, and CGRP-immunoreactive neurons amounted to 68%, 48%, 33%, and 54% in DRGs and 64%, 49%, 40%, and 25% in the NGs. Of the labeled DRG and NG neurons, 23% and 35% showed both TRPV1 and InsR immunoreactivity. Colocalization of the InsR with SP or CGRP was demonstrated in 14% and 28% of pancreatic DRG and 24% and 8% of pancreatic NG neurons. Conclusions The present findings provide morphological basis for possible functional interactions among the nociceptive ion channel TRPV1, the InsR, and the proinflammatory neuropeptides SP and CGRP expressed by pancreatic DRG and NG neurons.

Original languageEnglish
Pages (from-to)110-115
Number of pages6
JournalPancreas
Volume47
Issue number1
DOIs
Publication statusPublished - Jan 1 2018

Keywords

  • bWGA - biotin-conjugated wheat germ agglutinin
  • CGRP - calcitonin gene-related peptide
  • DRG - dorsal root ganglion
  • IGF-I - insulinlike growth factor 1
  • InsR - insulin receptor
  • insulin receptor
  • neuropeptides
  • pancreas
  • primary afferent neurons
  • retrograde labeling
  • SP - substance P
  • TRPV1
  • TRPV1 - transient receptor potential vanilloid type 1 receptor

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

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