The inhibitory action of exo- and endocannabinoids on [ 3H]GABA release are mediated by both CB 1 and CB 2 receptors in the mouse hippocampus

Rómeó D. Andó, Judit Bíró, Cecília Csölle, Catherine Ledent, Beáta Sperlágh

Research output: Contribution to journalArticle

23 Citations (Scopus)


Exogenous and endogenous cannabinoids play an important role in modulating the release of neurotransmitters in hippocampal excitatory and inhibitory networks, thus having profound effect on higher cognitive and emotional functions such as learning and memory. In this study we have studied the effect of cannabinoid agonists on the potassium depolarization-evoked [ 3H]GABA release from hippocampal synaptosomes in the wild-type (WT) and cannabinoid 1 receptor (CB 1R)-null mutant mice. All tested cannabinoid agonists (WIN55,212-2, CP55,940, HU-210, 2-arachidonoyl-glycerol, 2-AG; delta-9-tetra-hydrocannabinol, THC) inhibited [ 3H]GABA release in WT mice with the following rank order of agonist potency: HU-210 > CP55,490 > WIN55,212-2 >> 2-AG > THC. By contrast, 2-AG and THC displayed the greatest efficacy eliciting almost complete inhibition of evoked [ 3H]GABA efflux, whereas the maximal inhibition obtained by HU-210, CP55,490, and WIN55,212-2 were less, eliciting not more than 40% inhibition. The inhibitory effect of WIN55,212-2, THC and 2-AG on evoked [ 3H]GABA efflux was antagonized by the CB 1 receptor inverse agonist AM251 (0.5 μM) in the WT mice. In the CB 1R knockout mice the inhibitory effects of all three agonists were attenuated. In these mice, AM251 did not antagonize, but further reduced the [ 3H]GABA release in the presence of the synthetic agonist WIN55,212-2. By contrast, the concentration-dependent inhibitory effects of THC and 2-AG were partially antagonized by AM251 in the absence of CB 1 receptors. Finally, the inhibition of evoked [ 3H]GABA efflux by THC and 2-AG was also partially attenuated by AM630 (1 μM), the CB 2 receptor-selective antagonist, both in WT and CB 1 knockout mice. Our data prove the involvement of CB 1 receptors in the effect of exo- and endocannabinoids on GABA efflux from hippocampal nerve terminals. In addition, in the effect of the exocannabinoid THC and the endocannabinoid 2-AG, non-CB 1, probably CB 2-like receptors are also involved.

Original languageEnglish
Pages (from-to)145-152
Number of pages8
JournalNeurochemistry international
Issue number2
Publication statusPublished - Jan 1 2012


  • CB receptor
  • CB receptor
  • Cannabinoid
  • GABA
  • Hippocampus
  • Release

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

Fingerprint Dive into the research topics of 'The inhibitory action of exo- and endocannabinoids on [ <sup>3</sup>H]GABA release are mediated by both CB <sub>1</sub> and CB <sub>2</sub> receptors in the mouse hippocampus'. Together they form a unique fingerprint.

  • Cite this