The influence of salsolinol on dopaminergic system activity within the mediobasal hypothalamus of anestrous sheep

A model for studies on the salsolinol-dopamine relationship

Tomasz Misztal, Malgorzata Hasiec, Dorota Tomaszewska-Zaremba, Elzbieta Dobek, F. Fülöp, Katarzyna Romanowicz

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Salsolinol with its derivatives has been considered as a potential neurotoxin for the dopaminergic system in the human and rat brain. Investigating a sheep model for studies on the action of salsolinol within the central nervous system we examined whether this compound is able to affect the hypothalamic neuroendocrine dopaminergic (NEDA) system during its high seasonal activity, when sheep entered to anestrus under the long day conditions. Therefore, salsolinol was infused into the third ventricle of the brain in combination with the in vivo push-pull perfusion of the mediobasal hypothalamus/median eminence (MBH/ME). The effects of this drug on either perfusate noradrenaline (NA) or plasma prolactin concentration were also studied. The infusion of salsolinol resulted in rapid and permanent diminution in dopamine (DA) release into the extracellular spaces of the MBH/ME up to an undetectable level and in the 57% decrease in DA metabolite 3,4- dihydroxyphenylacetic acid concentration, compared to the control. This effect of salsolinol was accompanied by the significant enhancement of the pituitary prolactin release into circulation. The concentration of other DA metabolite, homovanillic acid, as well as NA in the MBH/ME was not affected. Thus, our results in the anestrous sheep underline the role played by salsolinol as a neuromodulator for the hypothalamic NEDA system and as a signal transmitter for the pituitary prolactin release. We suggest that the hypothalamic NEDA system of anestrous sheep during its high secretory activity may be set as a model for studies on the salsolinol-dopamine relationship.

Original languageEnglish
Pages (from-to)305-312
Number of pages8
JournalActa Neurobiologiae Experimentalis
Volume71
Issue number3
Publication statusPublished - 2011

Fingerprint

Hypothalamus
Dopamine
Sheep
Median Eminence
Neurosecretory Systems
Prolactin
Norepinephrine
Anestrus
3,4-Dihydroxyphenylacetic Acid
Homovanillic Acid
Third Ventricle
Neurotoxins
Brain
Extracellular Space
salsolinol
Neurotransmitter Agents
Central Nervous System
Perfusion
Pharmaceutical Preparations

Keywords

  • Anestrous sheep
  • Dopamine
  • Dopaminergic system
  • Hypothalamus
  • Prolactin release
  • Salsolinol

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The influence of salsolinol on dopaminergic system activity within the mediobasal hypothalamus of anestrous sheep : A model for studies on the salsolinol-dopamine relationship. / Misztal, Tomasz; Hasiec, Malgorzata; Tomaszewska-Zaremba, Dorota; Dobek, Elzbieta; Fülöp, F.; Romanowicz, Katarzyna.

In: Acta Neurobiologiae Experimentalis, Vol. 71, No. 3, 2011, p. 305-312.

Research output: Contribution to journalArticle

Misztal, Tomasz ; Hasiec, Malgorzata ; Tomaszewska-Zaremba, Dorota ; Dobek, Elzbieta ; Fülöp, F. ; Romanowicz, Katarzyna. / The influence of salsolinol on dopaminergic system activity within the mediobasal hypothalamus of anestrous sheep : A model for studies on the salsolinol-dopamine relationship. In: Acta Neurobiologiae Experimentalis. 2011 ; Vol. 71, No. 3. pp. 305-312.
@article{a454e335603e4bb1a0f7f797d0f26f40,
title = "The influence of salsolinol on dopaminergic system activity within the mediobasal hypothalamus of anestrous sheep: A model for studies on the salsolinol-dopamine relationship",
abstract = "Salsolinol with its derivatives has been considered as a potential neurotoxin for the dopaminergic system in the human and rat brain. Investigating a sheep model for studies on the action of salsolinol within the central nervous system we examined whether this compound is able to affect the hypothalamic neuroendocrine dopaminergic (NEDA) system during its high seasonal activity, when sheep entered to anestrus under the long day conditions. Therefore, salsolinol was infused into the third ventricle of the brain in combination with the in vivo push-pull perfusion of the mediobasal hypothalamus/median eminence (MBH/ME). The effects of this drug on either perfusate noradrenaline (NA) or plasma prolactin concentration were also studied. The infusion of salsolinol resulted in rapid and permanent diminution in dopamine (DA) release into the extracellular spaces of the MBH/ME up to an undetectable level and in the 57{\%} decrease in DA metabolite 3,4- dihydroxyphenylacetic acid concentration, compared to the control. This effect of salsolinol was accompanied by the significant enhancement of the pituitary prolactin release into circulation. The concentration of other DA metabolite, homovanillic acid, as well as NA in the MBH/ME was not affected. Thus, our results in the anestrous sheep underline the role played by salsolinol as a neuromodulator for the hypothalamic NEDA system and as a signal transmitter for the pituitary prolactin release. We suggest that the hypothalamic NEDA system of anestrous sheep during its high secretory activity may be set as a model for studies on the salsolinol-dopamine relationship.",
keywords = "Anestrous sheep, Dopamine, Dopaminergic system, Hypothalamus, Prolactin release, Salsolinol",
author = "Tomasz Misztal and Malgorzata Hasiec and Dorota Tomaszewska-Zaremba and Elzbieta Dobek and F. F{\"u}l{\"o}p and Katarzyna Romanowicz",
year = "2011",
language = "English",
volume = "71",
pages = "305--312",
journal = "Acta Neurobiologiae Experimentalis",
issn = "0065-1400",
publisher = "Nencki Institute of Experimental Biology",
number = "3",

}

TY - JOUR

T1 - The influence of salsolinol on dopaminergic system activity within the mediobasal hypothalamus of anestrous sheep

T2 - A model for studies on the salsolinol-dopamine relationship

AU - Misztal, Tomasz

AU - Hasiec, Malgorzata

AU - Tomaszewska-Zaremba, Dorota

AU - Dobek, Elzbieta

AU - Fülöp, F.

AU - Romanowicz, Katarzyna

PY - 2011

Y1 - 2011

N2 - Salsolinol with its derivatives has been considered as a potential neurotoxin for the dopaminergic system in the human and rat brain. Investigating a sheep model for studies on the action of salsolinol within the central nervous system we examined whether this compound is able to affect the hypothalamic neuroendocrine dopaminergic (NEDA) system during its high seasonal activity, when sheep entered to anestrus under the long day conditions. Therefore, salsolinol was infused into the third ventricle of the brain in combination with the in vivo push-pull perfusion of the mediobasal hypothalamus/median eminence (MBH/ME). The effects of this drug on either perfusate noradrenaline (NA) or plasma prolactin concentration were also studied. The infusion of salsolinol resulted in rapid and permanent diminution in dopamine (DA) release into the extracellular spaces of the MBH/ME up to an undetectable level and in the 57% decrease in DA metabolite 3,4- dihydroxyphenylacetic acid concentration, compared to the control. This effect of salsolinol was accompanied by the significant enhancement of the pituitary prolactin release into circulation. The concentration of other DA metabolite, homovanillic acid, as well as NA in the MBH/ME was not affected. Thus, our results in the anestrous sheep underline the role played by salsolinol as a neuromodulator for the hypothalamic NEDA system and as a signal transmitter for the pituitary prolactin release. We suggest that the hypothalamic NEDA system of anestrous sheep during its high secretory activity may be set as a model for studies on the salsolinol-dopamine relationship.

AB - Salsolinol with its derivatives has been considered as a potential neurotoxin for the dopaminergic system in the human and rat brain. Investigating a sheep model for studies on the action of salsolinol within the central nervous system we examined whether this compound is able to affect the hypothalamic neuroendocrine dopaminergic (NEDA) system during its high seasonal activity, when sheep entered to anestrus under the long day conditions. Therefore, salsolinol was infused into the third ventricle of the brain in combination with the in vivo push-pull perfusion of the mediobasal hypothalamus/median eminence (MBH/ME). The effects of this drug on either perfusate noradrenaline (NA) or plasma prolactin concentration were also studied. The infusion of salsolinol resulted in rapid and permanent diminution in dopamine (DA) release into the extracellular spaces of the MBH/ME up to an undetectable level and in the 57% decrease in DA metabolite 3,4- dihydroxyphenylacetic acid concentration, compared to the control. This effect of salsolinol was accompanied by the significant enhancement of the pituitary prolactin release into circulation. The concentration of other DA metabolite, homovanillic acid, as well as NA in the MBH/ME was not affected. Thus, our results in the anestrous sheep underline the role played by salsolinol as a neuromodulator for the hypothalamic NEDA system and as a signal transmitter for the pituitary prolactin release. We suggest that the hypothalamic NEDA system of anestrous sheep during its high secretory activity may be set as a model for studies on the salsolinol-dopamine relationship.

KW - Anestrous sheep

KW - Dopamine

KW - Dopaminergic system

KW - Hypothalamus

KW - Prolactin release

KW - Salsolinol

UR - http://www.scopus.com/inward/record.url?scp=83455225137&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=83455225137&partnerID=8YFLogxK

M3 - Article

VL - 71

SP - 305

EP - 312

JO - Acta Neurobiologiae Experimentalis

JF - Acta Neurobiologiae Experimentalis

SN - 0065-1400

IS - 3

ER -