The influence of methylated septin 9 gene on RNA and protein level in colorectal cancer

Kinga Tóth, O. Galamb, S. Spisák, Barnabás Wichmann, F. Sípos, Gábor Valcz, Katalin Leiszter, B. Molnár, Z. Tulassay

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Colorectal cancer is one of the leading death causes in the world. Specificity and sensitivity of the present screening methods are unsuitable and their compliance is too low. Nowadays the most effective method is the colonoscopy, because it gives not only macroscopic diagnosis but therapeutic possibility as well, however the compliance of the patients is very low. Hence development of new diagnostic methods is needed. Altered expression of septin 9 was found in several tumor types including colorectal cancer. The aim of this study was to detect the methylation related mRNA and protein expression changes of septin 9 in colorectal adenoma-dysplasia-carcinoma sequence and to analyze its reversibility by demethylation treatment. Septin 9 protein expression showed significant difference between normal and colorectal cancer (CRC) samples (p <0,001). According to biopsy microarray results, septin 9 mRNA expression decreased in the progression of colon neoplastic disease (p <0,001). In laser microdissected epithelial cells, septin 9 significantly underexpressed in CRC compared to healthy controls (p <0,001). The expression of septin9-v1 region was higher in the healthy samples, while septin9-v2, v4, v4*, v5 overexpression were detected in cancer epithelial cells compared to normal. The septin 9 mRNA and protein levels of HT29 cells increased after demethylation treatment. The increasing methylation of septin 9 gene during colorectal adenoma-dysplasia-carcinoma sequence progression is reflected in the decreasing mRNA and protein expression, especially in the epithelium. These changes can be reversed by demethylation agents converting this screening marker gene into therapeutic target.

Original languageEnglish
Pages (from-to)503-509
Number of pages7
JournalPathology and Oncology Research
Volume17
Issue number3
DOIs
Publication statusPublished - Sep 2011

Fingerprint

Septins
Colorectal Neoplasms
RNA
Genes
Proteins
Messenger RNA
Adenoma
Methylation
Epithelial Cells
Carcinoma
HT29 Cells
Colonoscopy
Patient Compliance
Compliance
Sequence Analysis
Cause of Death
Neoplasms
Colon
Lasers
Epithelium

Keywords

  • Colorectal cancer
  • mRNA expression
  • Peripheral blood
  • Protein expression
  • Septin 9

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pathology and Forensic Medicine

Cite this

The influence of methylated septin 9 gene on RNA and protein level in colorectal cancer. / Tóth, Kinga; Galamb, O.; Spisák, S.; Wichmann, Barnabás; Sípos, F.; Valcz, Gábor; Leiszter, Katalin; Molnár, B.; Tulassay, Z.

In: Pathology and Oncology Research, Vol. 17, No. 3, 09.2011, p. 503-509.

Research output: Contribution to journalArticle

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