The influence of amino acids on the lobeline production of lobelia inflata L. hairy root cultures

I. Bálványos, E. Szőke, L. Kursinszki

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The herb Lobelia inflata L. (Lobeliaceae) contains piperidine alkaloids. The main alkaloid is the pharmacologically-active lobeline. We have studied the effects of alkaloid precursor amino acids (lysine and phenylalanine) on the growth and alkaloid production of hairy root cultures of Lobelia inflata L. The hairy root clone 8009/h7 transformed with Agrobacterium rhizogenes strain R 1601 was cultivated on B5 solid medium containing lysine (Lys) and phenylalanine (Phe) both in the presence and absence of the growth regulators IAA and kinetin. On the medium containing hormones growth was delayed until day 14. The application of growth regulators to the B5 media containing amino acids either singly or in combination increased the biomass in all cases. The maximum dry weight was achieved in a medium containing Phe + Lys and growth regulators. The highest lobeline level (36 μg/g) was detected in tissues cultivated on hormone-free medium containing Phe. In hormone-free media, lobeline production increased in the presence of either Phe or Lys compared with the control, but the addition of both greatly decreased synthesis. In contrast, the addition of both amino acids to media supplemented with IAA and kinetin increased lobeline production.

Original languageEnglish
Pages (from-to)241-244
Number of pages4
JournalPlant Growth Regulation
Volume36
Issue number3
DOIs
Publication statusPublished - Mar 2002

Fingerprint

Lobelia
Lobelia inflata
Lobeline
Phenylalanine
phenylalanine
Alkaloids
Lysine
lysine
Amino Acids
amino acids
growth regulators
Kinetin
alkaloids
Growth
kinetin
indole acetic acid
piperidine alkaloids
hormones
Hormones
Campanulaceae

Keywords

  • Amino acids
  • Growth regulators
  • Hairy root
  • Lobelia inflata
  • Lobeline

ASJC Scopus subject areas

  • Plant Science

Cite this

The influence of amino acids on the lobeline production of lobelia inflata L. hairy root cultures. / Bálványos, I.; Szőke, E.; Kursinszki, L.

In: Plant Growth Regulation, Vol. 36, No. 3, 03.2002, p. 241-244.

Research output: Contribution to journalArticle

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