The importance of the PD-1/PD-L1 pathway at the maternal-fetal interface 11 Medical and Health Sciences 1107 Immunology

Matyas Meggyes, Eva Miko, Brigitta Szigeti, Nelli Farkas, L. Szereday

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Our goal with this study was to investigate the contribution of PD-1/PD-L1 immune-checkpoint pathway to maternal immunotolerance mechanisms. Methods: Thirteen healthy pregnant women and 10 non-pregnant controls were involved in this project. PBMCs and DICs were isolated from peripheral blood and from decidual tissues. After the characterization of different immune cell subsets, we used fluorochrome-conjugated monoclonal antibodies to measure the expression level of PD-1, PD-L1, NKG2D, and CD107a molecules by flow cytometry. Results: We measured significant alternations in the proportion of decidual immune cell subsets compared to the periphery. Elevated PD-1 expression by decidual CD8+ T, CD4+ T, and NKT-like cells were also detected accompanied by the increased PD-L1 expression by decidual CD4+ T, Treg, NKT-like and CD56 + NK cell subsets compared to peripheral blood. The cytotoxic potential was significantly higher in PD-1- decidual immune cells compared to the periphery, however we measured a significantly lower cytotoxicity in the decidual PD-1+ CD8+ T cells compared with the peripheral subsets. An activation receptor NKG2D expression was decreased by the PD-1+ CD8+ T subsets in the first trimester compared to non-pregnant condition but the expression level of the decidual counterparts was significantly elevated compared to the periphery. The cytotoxic potential of decidual PD1/NKG2D double positive CD8+ T cells was significantly decreased compared to the peripheral subsets. Conclusions: Based on our results we assume that PD-1/PD-L1 pathway might have a novel role in the maintaining of the local immunological environment. Accompanied by NKG2D activating receptor this checkpoint interaction could regulate decidual CD8 Tc cell subsets and may contribute maternal immunotolerance.

Original languageEnglish
Article number74
JournalBMC Pregnancy and Childbirth
Volume19
Issue number1
DOIs
Publication statusPublished - Feb 19 2019

Fingerprint

Allergy and Immunology
NK Cell Lectin-Like Receptor Subfamily K
Mothers
Health
T-Lymphocytes
Dacarbazine
Natural Killer T-Cells
First Pregnancy Trimester
Fluorescent Dyes
Natural Killer Cells
Pregnant Women
Flow Cytometry
Monoclonal Antibodies

Keywords

  • Maternal immunotolerance
  • Maternal-fetal interface
  • PD-1
  • PD-L1
  • Pregnancy

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

The importance of the PD-1/PD-L1 pathway at the maternal-fetal interface 11 Medical and Health Sciences 1107 Immunology. / Meggyes, Matyas; Miko, Eva; Szigeti, Brigitta; Farkas, Nelli; Szereday, L.

In: BMC Pregnancy and Childbirth, Vol. 19, No. 1, 74, 19.02.2019.

Research output: Contribution to journalArticle

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