The aims of the investigation were 1. to determine the location and condition of the spindle with the "spindle view" technique during quality control of the human oocytes and 2. to study the effect of low temperature to the detect-ability and condition of the spindle in MII mouse eggs. The position of the spindle was determined in 124 MII human oocytes. In 62% of the oocytes (77/124) the spindle was located at 6 or 12 o'clock, in 25% of the oocytes (31/124) at 3 or 9 o'clock and in 10.5% of the cases it was detected in the polar body (13/124). In 2.5% of the eggs (3/124) no polar body was present but we were able to detect the spindle. No difference was found in the fertilization rate of the experimental (polscope +) and the control (polscope -) group of oocytes (89/124; 72% vs. 62/94; 66%). Our results indicate that the polscope technique has no detrimental effect to the fertilization and development of oocytes/embryos. Our observations show that the sub-optimal temperature treatment induced changes in the structure of the spindle. In 80% of the treated oocytes (16/20) the spindle could be detected immediately after treatment. This ratio was not different from that of the untreated group (21/24; 87%). However, the spindle could be detected only in 25% of the eggs (5/20) when the spindle visualization was repeated 30 min after low temperature treatment. The "spindle view" technique, together with the traditional evaluation of oocyte quality based on morphology, can make the selection of eggs safer in the future. Oocytes with better quality have more chance for fertilization, development and implantation. Furthermore, with the "spindle view" technique, the location of the spindle can be determined and we can avoid the damage of the chromosomes located on the spindle during ICSI.
|Number of pages||7|
|Journal||Magyar Noorvosok Lapja|
|Publication status||Published - Mar 31 2004|
ASJC Scopus subject areas
- Reproductive Medicine
- Obstetrics and Gynaecology