The importance of microenvironment: The role of CCL8 in metastasis formation of melanoma

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We have attempted to characterize the changes occurring on the host side during the progression of human melanoma. To investigate the role of tumor microenvironment, we set up such an animal model, which was able to isolate the host related factors playing central role in metastasis formation. One of these 'factors', CCL12, was consequently selected and its behavior was examined alongside its human homologue (CCL8). In our animal model, metastasis forming primary melanoma in the host exhibited increased level of CCL12 mRNA expression. In clinical samples, when examining the tumor and the host together, the cumulative (tumor and host) CCL8 expression was lower in the group in which human primary melanoma formed lung metastasis compared to non-metastatic primary tumors. We could not detect significant difference in CCL8 receptor (CCR1) expression between the two groups. Increased migration of the examined tumor cell lines was observed when CCL8 was applied as a chemoattractant. The tumor cells and their interactions can be influenced the expression of CCL8 by dermal fibroblasts, as a significant change in the metastatic microenvironment. Furthermore, we examined changes in miRNA profile resulted by CCL8 and miR146a appears to be a promising prognostic marker for following this process.

Original languageEnglish
Pages (from-to)29111-29128
Number of pages18
JournalOncotarget
Volume6
Issue number30
DOIs
Publication statusPublished - 2015

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Melanoma
Neoplasm Metastasis
CCR1 Receptors
Animal Models
Neoplasms
Tumor Microenvironment
Chemotactic Factors
Tumor Cell Line
MicroRNAs
Cell Communication
Fibroblasts
Lung
Messenger RNA
Skin

Keywords

  • CCL8
  • Melanoma metastasis
  • Microenvironment
  • MiR146a

ASJC Scopus subject areas

  • Oncology

Cite this

The importance of microenvironment : The role of CCL8 in metastasis formation of melanoma. / Barbai, Tamás; Fejös, Z.; Puskás, L.; Tímár, J.; Rásó, E.

In: Oncotarget, Vol. 6, No. 30, 2015, p. 29111-29128.

Research output: Contribution to journalArticle

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