The immunophenotype of osteoclasts and macrophage polykaryons

F. Maggiani, R. Forsyth, P. C W Hogendoorn, T. Krenács, N. A. Athanasou

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Aim: Osteoclasts are multinucleated cells which are specialised to carry out lacunar bone resorption. Osteoclasts form part of the mononuclear phagocyte system, and immunophenotypic criteria for distinction from macrophage polykaryons include expression of CD51 (vitronectin receptor) and absence of HLA-DR and CD14. Methods: The expression of CD14, CD163, HLA-DR and CD51 in formalin-fixed paraffin-embedded sections of normal bone and neoplastic and non-neoplastic lesions of bone and soft tissue known to contain osteoclasts and macrophage polykaryons respectively was assessed immunohistochemically; the immunophenotype of osteoclast-like giant cells in a wide range of giant cell-containing bone lesions was similarly assessed. Results: Both osteoclasts and macrophage polykaryons were found to express CD51. Macrophage polykaryons, but not osteoclasts, expressed CD14 and HLA-DR. CD51+/CD14-/HLA-DR-/CD163- giant cells were noted in all giant-cell lesions of bone, including giant cell tumour of bone, aneurysmal bone cyst, non-ossifying fibroma, chondroblastoma, telangiectatic osteosarcoma, chondromyxoid fibroma, Langerhans cell histiocytosis and brown tumour. Conclusion: Our findings indicate that CD51 expression alone is not sufficient for immunocytochemical identification of osteoclasts, which do not express the macrophage-associated antigens CD14 and HLA-DR. Giant cells in most giant cell-rich lesions of bone have an osteoclast phenotype, suggesting that they are formed from mononuclear phagocyte osteoclast precursors.

Original languageEnglish
Pages (from-to)701-705
Number of pages5
JournalJournal of Clinical Pathology
Volume64
Issue number8
DOIs
Publication statusPublished - Aug 2011

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Osteoclasts
Macrophages
Giant Cells
HLA-DR Antigens
Bone and Bones
Fibroma
Chondroblastoma
Integrin alphaVbeta3
Giant Cell Tumor of Bone
Aneurysmal Bone Cysts
Langerhans Cell Histiocytosis
Mononuclear Phagocyte System
Osteosarcoma
Phagocytes
Bone Resorption
Paraffin
Formaldehyde
Phenotype

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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Maggiani, F., Forsyth, R., Hogendoorn, P. C. W., Krenács, T., & Athanasou, N. A. (2011). The immunophenotype of osteoclasts and macrophage polykaryons. Journal of Clinical Pathology, 64(8), 701-705. https://doi.org/10.1136/jcp.2011.090852

The immunophenotype of osteoclasts and macrophage polykaryons. / Maggiani, F.; Forsyth, R.; Hogendoorn, P. C W; Krenács, T.; Athanasou, N. A.

In: Journal of Clinical Pathology, Vol. 64, No. 8, 08.2011, p. 701-705.

Research output: Contribution to journalArticle

Maggiani, F, Forsyth, R, Hogendoorn, PCW, Krenács, T & Athanasou, NA 2011, 'The immunophenotype of osteoclasts and macrophage polykaryons', Journal of Clinical Pathology, vol. 64, no. 8, pp. 701-705. https://doi.org/10.1136/jcp.2011.090852
Maggiani, F. ; Forsyth, R. ; Hogendoorn, P. C W ; Krenács, T. ; Athanasou, N. A. / The immunophenotype of osteoclasts and macrophage polykaryons. In: Journal of Clinical Pathology. 2011 ; Vol. 64, No. 8. pp. 701-705.
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