The Hypocrea jecorina gal10 (uridine 5′-diphosphate-glucose 4-epimerase-encoding) gene differs from yeast homologues in structure, genomic organization and expression

Bernhard Seiboth, L. Karaffa, E. Sándor, Christian P. Kubicek

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

As part of a comprehensive study on lactose metabolism in Hypocrea jecorina (anamorph: Trichoderma reesei), a genomic clone of the gal10 gene encoding H. jecorina uridine 5′-diphosphate (UDP)-glucose 4-epimerase has been cloned and sequenced. It contains an open reading frame of 1548-base pair, interrupted by three introns, and encoding a 370-amino acids protein with similarity to pro- and eukaryotic UDP-glucose-4-epimerases. H. jecorina Gal10 does not contain the C-terminal mutarotase domain which is present in yeast Gal10 proteins but is able to functionally complement a corresponding Saccharomyces cerevisiae gal10 mutant. gal10 is not clustered with other H. jecorina gal genes (gal7, gene encoding galactose-1-phosphate uridylyltransferase and gal1, gene encoding galactokinase). The genomic location of H. jecorina gal10 and gal7 was syntenic with that in Neurospora crassa and colinear over an area of 6 and 3.5-kilobase. gal10 is constitutively expressed, and - unlike H. jecorina gal7 - not further stimulated by D-galactose or L-arabinose or its corresponding polyols.

Original languageEnglish
Pages (from-to)143-149
Number of pages7
JournalGene
Volume295
Issue number1
DOIs
Publication statusPublished - Jul 24 2002

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Hypocrea
Uridine Diphosphate Glucose
Racemases and Epimerases
Yeasts
Genes
Galactokinase
Neurospora crassa
Arabinose
Trichoderma
Fungal Proteins
Lactose
Galactose
Base Pairing
Introns
Open Reading Frames
Saccharomyces cerevisiae
Clone Cells
Amino Acids
Proteins

Keywords

  • Galactose
  • Intron
  • Lactose
  • Neurospora
  • Synteny
  • Trichoderma reesei

ASJC Scopus subject areas

  • Genetics

Cite this

The Hypocrea jecorina gal10 (uridine 5′-diphosphate-glucose 4-epimerase-encoding) gene differs from yeast homologues in structure, genomic organization and expression. / Seiboth, Bernhard; Karaffa, L.; Sándor, E.; Kubicek, Christian P.

In: Gene, Vol. 295, No. 1, 24.07.2002, p. 143-149.

Research output: Contribution to journalArticle

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abstract = "As part of a comprehensive study on lactose metabolism in Hypocrea jecorina (anamorph: Trichoderma reesei), a genomic clone of the gal10 gene encoding H. jecorina uridine 5′-diphosphate (UDP)-glucose 4-epimerase has been cloned and sequenced. It contains an open reading frame of 1548-base pair, interrupted by three introns, and encoding a 370-amino acids protein with similarity to pro- and eukaryotic UDP-glucose-4-epimerases. H. jecorina Gal10 does not contain the C-terminal mutarotase domain which is present in yeast Gal10 proteins but is able to functionally complement a corresponding Saccharomyces cerevisiae gal10 mutant. gal10 is not clustered with other H. jecorina gal genes (gal7, gene encoding galactose-1-phosphate uridylyltransferase and gal1, gene encoding galactokinase). The genomic location of H. jecorina gal10 and gal7 was syntenic with that in Neurospora crassa and colinear over an area of 6 and 3.5-kilobase. gal10 is constitutively expressed, and - unlike H. jecorina gal7 - not further stimulated by D-galactose or L-arabinose or its corresponding polyols.",
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