The Hypocrea jecorina gal10 (uridine 5′-diphosphate-glucose 4-epimerase-encoding) gene differs from yeast homologues in structure, genomic organization and expression

Bernhard Seiboth, Levente Karaffa, Erzsébet Sándor, Christian P. Kubicek

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

As part of a comprehensive study on lactose metabolism in Hypocrea jecorina (anamorph: Trichoderma reesei), a genomic clone of the gal10 gene encoding H. jecorina uridine 5′-diphosphate (UDP)-glucose 4-epimerase has been cloned and sequenced. It contains an open reading frame of 1548-base pair, interrupted by three introns, and encoding a 370-amino acids protein with similarity to pro- and eukaryotic UDP-glucose-4-epimerases. H. jecorina Gal10 does not contain the C-terminal mutarotase domain which is present in yeast Gal10 proteins but is able to functionally complement a corresponding Saccharomyces cerevisiae gal10 mutant. gal10 is not clustered with other H. jecorina gal genes (gal7, gene encoding galactose-1-phosphate uridylyltransferase and gal1, gene encoding galactokinase). The genomic location of H. jecorina gal10 and gal7 was syntenic with that in Neurospora crassa and colinear over an area of 6 and 3.5-kilobase. gal10 is constitutively expressed, and - unlike H. jecorina gal7 - not further stimulated by D-galactose or L-arabinose or its corresponding polyols.

Original languageEnglish
Pages (from-to)143-149
Number of pages7
JournalGene
Volume295
Issue number1
DOIs
Publication statusPublished - Jul 24 2002

Keywords

  • Galactose
  • Intron
  • Lactose
  • Neurospora
  • Synteny
  • Trichoderma reesei

ASJC Scopus subject areas

  • Genetics

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