The homolog of the five SH3-domain protein (HOFI/SH3PXD2B) regulates lamellipodia formation and cell spreading

Árpád Lányi, Mónika Baráth, Zalán Péterfi, Gábor Bögel, Anna Orient, Tünde Simon, Enikö Petrovszki, Katalin Kis-Tóth, Gábor Sirokmány, Éva Rajnavölgyi, Cox Terhorst, László Buday, Miklós Geiszt

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20 Citations (Scopus)


Motility of normal and transformed cells within and across tissues requires specialized subcellular structures, e.g. membrane ruffles, lamellipodia and podosomes, which are generated by dynamic rearrangements of the actin cytoskeleton. Because the formation of these sub-cellular structures is complex and relatively poorly understood, we evaluated the role of the adapter protein SH3PXD2B [HOFI, fad49, Tks4], which plays a role in the development of the eye, skeleton and adipose tissue. Surprisingly, we find that SH3PXD2B is requisite for the development of EGF-induced membrane ruffles and lamellipodia, as well as for efficient cellular attachment and spreading of HeLa cells. Furthermore, SH3PXD2B is present in a complex with the non-receptor protein tyrosine kinase Src, phosphorylated by Src, which is consistent with SH3PXD2B accumulating in Src-induced podosomes. Furthermore, SH3PXD2B closely follows the subcellular relocalization of cortactin to Src-induced podosomes, EGF-induced membrane ruffles and lamellipodia. Because SH3PXD2B also forms a complex with the C-terminal region of cortactin, we propose that SH3PXD2B is a scaffold protein that plays a key role in regulating the actin cytoskeleton via Src and cortactin.

Original languageEnglish
Article numbere23653
JournalPloS one
Issue number8
Publication statusPublished - Aug 23 2011


ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Lányi, Á., Baráth, M., Péterfi, Z., Bögel, G., Orient, A., Simon, T., Petrovszki, E., Kis-Tóth, K., Sirokmány, G., Rajnavölgyi, É., Terhorst, C., Buday, L., & Geiszt, M. (2011). The homolog of the five SH3-domain protein (HOFI/SH3PXD2B) regulates lamellipodia formation and cell spreading. PloS one, 6(8), [e23653].