The histone phosphatase inhibitory property of plant nucleosome assembly protein-related proteins (NRPs)

Judit Bíró, Ilona Farkas, Mónika Domoki, Krisztina Ötvös, S. Bottka, V. Dombrádi, A. Fehér

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

SET/I2PP2A, a member of the family of nucleosome assembly proteins (NAPs), has been previously described as a multifunctional protein inhibiting protein phosphatase 2A (PP2A)-mediated histone H3(pSer10) dephosphorylation during the heat shock response in animal cells. In the present work we demonstrate that its plant orthologs, designated as NAP-related proteins (NRPs), have a similar invitro biochemical activity and interact with PP2A and histone H3(pSer10) invivo. Although heat shock gene promoters were found to be associated with histone H3(pSer10)-marked chromatin following a high temperature treatment, heat shock gene expression was not affected in NRP-deficient mutant Arabidopsis thaliana (L.) plantlets. These observations indicate that NRPs are potential regulators of histone dephosphorylation in plants, but they are dispensable for gene expression reorganization in response to heat shock.

Original languageEnglish
Pages (from-to)162-168
Number of pages7
JournalPlant Physiology and Biochemistry
Volume52
DOIs
Publication statusPublished - Mar 2012

Fingerprint

nucleosomes
Nucleosomes
Phosphoric Monoester Hydrolases
histones
Histones
Proteins
proteins
Protein Phosphatase 2
Heat-Shock Response
heat stress
phosphoprotein phosphatase
dephosphorylation
Shock
Hot Temperature
Gene Expression
Plant Genes
gene expression
Mutant Proteins
Arabidopsis
Chromatin

Keywords

  • Arabidopsis thaliana (L.)
  • Heat shock gene expression
  • Histone H3 dephosphorylation
  • Medicago sativa (L.)
  • Plant SET/I orthologue
  • Protein phosphatase 2A

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

The histone phosphatase inhibitory property of plant nucleosome assembly protein-related proteins (NRPs). / Bíró, Judit; Farkas, Ilona; Domoki, Mónika; Ötvös, Krisztina; Bottka, S.; Dombrádi, V.; Fehér, A.

In: Plant Physiology and Biochemistry, Vol. 52, 03.2012, p. 162-168.

Research output: Contribution to journalArticle

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AU - Fehér, A.

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