The high level of benomyl tolerance in Fusarium oxysporum is determined by the synergistic interaction of two genes

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Abstract

Strains with moderate benomyl tolerance [minimal inhibitory concentration (MIC) = 11.50-14.80 μg ml-1] were obtained by induced mutation from complementary auxotrophic mutants of Fusarium oxysporum f.sp. lycopersici. These strains were hybridized in different combinations by means of protoplast fusion. Among the hybrids, moderately resistant (parental-type), supertolerant (MIC>100 μg ml-1), and normal sensitive strains (MIC = 2.32 μg ml-1) were found. The presence of both supertolerant and normal sensitive strains indicates that a synergistic interaction of two genes is needed for a high level of tolerance. Strains which carried the same benomyl resistance gene failed to give supertolerant or normal sensitive hybrids. When sensitive and moderately tolerant strains were hybridized only parental phenotypes were present among the hybrids.

Original languageEnglish
Pages (from-to)326-333
Number of pages8
JournalExperimental Mycology
Volume9
Issue number4
DOIs
Publication statusPublished - Dec 1985

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Keywords

  • Fusarium oxysporum
  • benomyl tolerance
  • protoplast fusion
  • resistance genes
  • synergistic interaction

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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