The high-conductance channels of yeast mitochondrial outer membranes: A planar bilayer study

G. Báthori, Ildikò Szabò, Daniel Wolff, Mario Zoratti

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The high-conductance channels present in the outer membranes of wild-type and porin-less yeast mitochondria have been characterized electrophysiologically after incorporation in planar bilayer membranes. The most prominent activity was ascribed to a voltage-dependent, substate-rich, cationic channel which generally inactivated at potentials positive in the cis compartment, in agreement with the observations from patch-clamp experiments on porin-less mitoplasts. This channel has been identified as the so-called 'peptide-sensitive channel' (PSC). We also observed similar channels displaying either no inactivation, or inactivation at both positive and negative potentials. These latter properties match those already described for mammalian and yeast PSC, respectively. These different behaviors are tentatively explained as arising from the presence, or lack of, peptides bound to the PSC. Very high conductances, apparently due to cooperative gating, were frequently displayed. In wild-type membranes, activity ascribable to the porin was also observed.

Original languageEnglish
Pages (from-to)191-198
Number of pages8
JournalJournal of Bioenergetics and Biomembranes
Volume28
Issue number2
DOIs
Publication statusPublished - 1996

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Porins
Mitochondrial Membranes
Yeasts
Membranes
Mitochondria
Peptides
peptide sensitive channel

Keywords

  • Electrophysiology
  • mitochondrial megachannel
  • peptide-sensitive channel
  • planar bilayer
  • porin
  • yeast mitochondria

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Cite this

The high-conductance channels of yeast mitochondrial outer membranes : A planar bilayer study. / Báthori, G.; Szabò, Ildikò; Wolff, Daniel; Zoratti, Mario.

In: Journal of Bioenergetics and Biomembranes, Vol. 28, No. 2, 1996, p. 191-198.

Research output: Contribution to journalArticle

Báthori, G. ; Szabò, Ildikò ; Wolff, Daniel ; Zoratti, Mario. / The high-conductance channels of yeast mitochondrial outer membranes : A planar bilayer study. In: Journal of Bioenergetics and Biomembranes. 1996 ; Vol. 28, No. 2. pp. 191-198.
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