The high-conductance channel of porin-less yeast mitochondria

Ildikó Szabó, G. Báthori, Daniel Wolff, Tanja Starc, Carmen Cola, Mario Zoratti

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Patch-clamp and planar bilayer experiments on porin-less yeast mitochondria have allowed the characterization of a cationic channel activated at matrix-side positive (unphysiological) potentials. In voltage-pulse experiments, inactivation was a faster process than activation and the time constant for inactivation was more steeply dependent on voltage than the one for activation. The channel exhibited various conductance states whose occupancy depended on the applied transmembrane potential. In bilayer experiments, the presence of the pCOx-IV leader peptide induced fast gating in a voltage-dependent manner. A comparison with previously described activities suggests that the pore may coincide with the peptide-sensitive channel (PSC) (Thieffry et al. (1988) EMBO J. 7, 1449-1454) as well as with two other activities (Dibanich et al. (1989) Eur. J. Biochem. 181, 703-708; Tedeschi et al. (1987) J. Membr. Biol. 97, 21-29) assigned to the mitochondrial outer membrane. The possible relationship of this channel to the mitochondrial megachannel is discussed.

Original languageEnglish
Pages (from-to)115-125
Number of pages11
JournalBBA - Biomembranes
Volume1235
Issue number1
DOIs
Publication statusPublished - Apr 12 1995

Fingerprint

Porins
Mitochondria
Mitochondrial Membranes
Protein Sorting Signals
Membrane Potentials
Yeast
Yeasts
Electric potential
Chemical activation
Experiments
Clamping devices
Membranes
peptide sensitive channel
mitochondrial permeability transition pore

Keywords

  • (Yeast)
  • Patch clamp
  • Peptide-sensitive channel
  • Permeability transition
  • Porin-less mitochondrion

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

The high-conductance channel of porin-less yeast mitochondria. / Szabó, Ildikó; Báthori, G.; Wolff, Daniel; Starc, Tanja; Cola, Carmen; Zoratti, Mario.

In: BBA - Biomembranes, Vol. 1235, No. 1, 12.04.1995, p. 115-125.

Research output: Contribution to journalArticle

Szabó, I, Báthori, G, Wolff, D, Starc, T, Cola, C & Zoratti, M 1995, 'The high-conductance channel of porin-less yeast mitochondria', BBA - Biomembranes, vol. 1235, no. 1, pp. 115-125. https://doi.org/10.1016/0005-2736(94)00306-A
Szabó, Ildikó ; Báthori, G. ; Wolff, Daniel ; Starc, Tanja ; Cola, Carmen ; Zoratti, Mario. / The high-conductance channel of porin-less yeast mitochondria. In: BBA - Biomembranes. 1995 ; Vol. 1235, No. 1. pp. 115-125.
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