The heterogeneity of bovine IgG2. II. The identification of IgG2b

J. E. Butler, H. Heyermann, L. Frenyó, J. Kiernan

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Goat and rabbit polyclonal reagents can be raised which recognize a new isotype of bovine antibodies. The polyclonal goat reagent was raised against a preparation enriched in the major IgG2 isotype (IgG2a) which contained the new isotype as a contaminant. The polyclonal rabbit reagent was prepared against a trypsin-derived Fc fraction of bovine IgG1 which contained the Fc of the new isotype as a contaminant. This new isotype is present in the sera of the cattle of all breeds tested regardless of their IgG2a allotype and is antigenically distinct from IgG2a, IgG1, IgA, IgM and IgE. The new isotype coelutes from DEAE anion exchangers with IgG1 and the more acidic populations of IgG2a. The isotype is tentatively designated IgG2b. The distribution of IgG2b antibody activity to E. coli K99 and phosphorylcholine among 15 cattle of different A allotypes is not correlated with the IgG2a or IgG1 antibody activity in these animals.

Original languageEnglish
Pages (from-to)31-38
Number of pages8
JournalImmunology Letters
Volume16
Issue number1
DOIs
Publication statusPublished - 1987

Fingerprint

Immunoglobulin G
Goats
Antibodies
Rabbits
Phosphorylcholine
Trypsin
Immunoglobulin A
Immunoglobulin E
Anions
Immunoglobulin M
Escherichia coli
Serum
Population

Keywords

  • Bovine IgG2b

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

The heterogeneity of bovine IgG2. II. The identification of IgG2b. / Butler, J. E.; Heyermann, H.; Frenyó, L.; Kiernan, J.

In: Immunology Letters, Vol. 16, No. 1, 1987, p. 31-38.

Research output: Contribution to journalArticle

Butler, J. E. ; Heyermann, H. ; Frenyó, L. ; Kiernan, J. / The heterogeneity of bovine IgG2. II. The identification of IgG2b. In: Immunology Letters. 1987 ; Vol. 16, No. 1. pp. 31-38.
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