(matrix presented) Ammonium tert-butyl H-phosphonate was used for the phosphorylation of Tyr- and Ser-containing peptides synthesized by an Fmoc strategy. This reaction, leading to a monoprotected peptide phosphate, was found to be highly efficient and generally applicable. Moreover, the method employed avoids undesired side reactions during chain elongation (pyrophosphate formation and β-elimination catalyzed by piperidine).
ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Organic Chemistry