The genetic status of the Hungarian brown trout populations: Exploration of a blind spot on the European map of Salmo trutta studies

Ágnes Ősz, A. Horváth, György Hoitsy, Dóra Kánainé Sipos, Szilvia Keszte, Anna Júlia Sáfrány, Saša Marić, Csaba Palkó, Balázs Tóth, B. Urbányi, Balázs Kovács

Research output: Contribution to journalArticle

Abstract

Background. Analyses of the control region sequences of European brown trout populations’ mitrochondrial DNA have revealed five main evolutionary lineages (Atlantic, Danubian, Mediterranean, Adriatic, Marble) mostly relating to the main water basins; however, the hybridization between lineages were increasingly reported. Due to the hydrogeography of Hungary, wild populations should theoretically belong to the Danubian lineage, however, this has not been verified by genetic studies. Methods. In our study multiple molecular marker sets (mitochondrial sequence, microsatellites, PCR-RFLP of nuclear markers and sex marker) were used to investigate the genetic composition and population genetics of the brown trout populations in two broodstocks, six wild streams in Hungary and one Serbian population. Results. The admixture of Atlantic and Danubian lineages in these populations, except the Serbian population with pure Danubian origin, was observed by control region sequences of mitochondrial DNA and PCR-RFLP markers in the nuclear genome, and one unpublished Danubian haplotype was found in Hungarian populations. A sex-specific marker revealed equal gender ratio in broodstocks and Kemence stream, whereas in other wild streams the proportion of female individuals were less than 50%. Structure and principal component analyses based on the alleles of microsatellite loci also revealed overlapping populations, however the populations were still significantly different from each other and were mostly in Hardy-Weinberg equilibrium. Discussion. Stocking and migration can have a significant genetic impact on trout populations of wild streams, however there are no guidelines or common practices for stocking of small streams in Hungary, thus the genetic background of these populations should be considered when developing conservation actions.

Original languageEnglish
Article numbere5152
JournalPeerJ
Volume2018
Issue number9
DOIs
Publication statusPublished - Jan 1 2018

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Trout
Optic Disk
Salmo trutta
Microsatellite Repeats
Calcium Carbonate
Mitochondrial DNA
Population
Conservation
Genes
Hungary
Water
DNA
Chemical analysis
breeding stock
Restriction Fragment Length Polymorphisms
gender
restriction fragment length polymorphism
microsatellite repeats
Polymerase Chain Reaction
Population Genetics

Keywords

  • Hybridization
  • Lineage
  • Microsatellite
  • Mitochondrial DNA
  • Salmo trutta
  • Sex ratio

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

The genetic status of the Hungarian brown trout populations : Exploration of a blind spot on the European map of Salmo trutta studies. / Ősz, Ágnes; Horváth, A.; Hoitsy, György; Sipos, Dóra Kánainé; Keszte, Szilvia; Sáfrány, Anna Júlia; Marić, Saša; Palkó, Csaba; Tóth, Balázs; Urbányi, B.; Kovács, Balázs.

In: PeerJ, Vol. 2018, No. 9, e5152, 01.01.2018.

Research output: Contribution to journalArticle

Ősz, Á, Horváth, A, Hoitsy, G, Sipos, DK, Keszte, S, Sáfrány, AJ, Marić, S, Palkó, C, Tóth, B, Urbányi, B & Kovács, B 2018, 'The genetic status of the Hungarian brown trout populations: Exploration of a blind spot on the European map of Salmo trutta studies', PeerJ, vol. 2018, no. 9, e5152. https://doi.org/10.7717/peerj.5152
Ősz, Ágnes ; Horváth, A. ; Hoitsy, György ; Sipos, Dóra Kánainé ; Keszte, Szilvia ; Sáfrány, Anna Júlia ; Marić, Saša ; Palkó, Csaba ; Tóth, Balázs ; Urbányi, B. ; Kovács, Balázs. / The genetic status of the Hungarian brown trout populations : Exploration of a blind spot on the European map of Salmo trutta studies. In: PeerJ. 2018 ; Vol. 2018, No. 9.
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AU - Sipos, Dóra Kánainé

AU - Keszte, Szilvia

AU - Sáfrány, Anna Júlia

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