The genetic control of natural killer cell activity and its association with in vivo resistance against a moloney lymphoma isograft

G. Petrányi, Rolf Kiessling, Susan Povey, George Klein, Leonard Herzenberg, Hans Wigzell

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Abstract

Spleens of normal young mice of certain strains contain lymphocytes that can kill strain A-derived YAC-1 lymphoma cells in a51Cr release cytotoxic assay in vitro. We have previously classified mouse genotypes as high or low reactors, according to their responses in this test. In vivo resistance to small numbers of YAC ascites lymphoma cells is correlated with in vitro cytolytic activity. In vitro and in vivo tests were carried out on the same individual (A x C57BL)F1 x A backcross mice. Natural in vitro killer cell activity appeared to be under polygenic control, including a strong H-2-linked factor. No linkage was found with five different isozyme loci, with the Ig-l locus or with C5 serum activity. Also in vivo resistance showed strong linkage with the H-2 complex. In (A x CBA)F1 x A backcross mice, a weak linkage was found with the coat color locus C. There was a correlation between in vitro killer activity and in vivo resistance in the same backcross mice. In vivo resistance was particularly strong in mice that combined the H-2b-linked resistance factor with a high cytolytic activity in vitro.

Original languageEnglish
Pages (from-to)15-28
Number of pages14
JournalImmunogenetics
Volume3
Issue number1
DOIs
Publication statusPublished - Dec 1976

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Isografts
Natural Killer Cells
Lymphoma
R Factors
Ascites
Isoenzymes
In Vitro Techniques
Spleen
Color
Genotype
Lymphocytes
Serum

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

The genetic control of natural killer cell activity and its association with in vivo resistance against a moloney lymphoma isograft. / Petrányi, G.; Kiessling, Rolf; Povey, Susan; Klein, George; Herzenberg, Leonard; Wigzell, Hans.

In: Immunogenetics, Vol. 3, No. 1, 12.1976, p. 15-28.

Research output: Contribution to journalArticle

Petrányi, G. ; Kiessling, Rolf ; Povey, Susan ; Klein, George ; Herzenberg, Leonard ; Wigzell, Hans. / The genetic control of natural killer cell activity and its association with in vivo resistance against a moloney lymphoma isograft. In: Immunogenetics. 1976 ; Vol. 3, No. 1. pp. 15-28.
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